Human/Mouse GSK-3 alpha / beta Antibody

Catalog # Availability Size / Price Qty
MAB25063
MAB25063-SP
Human/Mouse GSK-3 alpha / beta  Antibody in Data
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Human/Mouse GSK-3 alpha / beta Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human GSK-3 beta in direct ELISAs and human GSK-3 alpha and GSK-3 beta in Western blots. Detects human and mouse GSK-3 alpha / beta in immunocytochemistry.
Source
Monoclonal Mouse IgG2B Clone # 833916
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GSK-3 beta
Asp200-Phe305
Accession # P49841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GSK 3 alpha  and GSK‑3 beta  by Western Blot. View Larger

Detection of Human GSK 3 alpha and GSK‑3 beta by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GSK-3a at approximately 46 and GSK-3 beta at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GSK‑3 alpha / beta  in HT‑29 Human Cell Line. View Larger

GSK‑3 alpha / beta in HT‑29 Human Cell Line. GSK-3a/ beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry GSK‑3 alpha / beta  in NIH‑3T3 Mouse Cell Line. View Larger

GSK‑3 alpha / beta in NIH‑3T3 Mouse Cell Line. GSK-3a/ beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Long Name
Glycogen Synthase Kinase 3
Alternate Names
DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK-3 alpha/beta

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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