Human/Mouse JunD Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse JunD by Western Blot.
1 Image
Product Details
Citations (2)
Supplemental Products

Human/Mouse JunD Antibody Summary

Species Reactivity
Human, Mouse
Detects endogenous human and mouse JunD in Western blots.
Monoclonal Mouse IgG2B Clone # 512709
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human JunD
Accession # CAA40010
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse JunD antibody by Western Blot. View Larger

Detection of Human/Mouse JunD by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse JunD Monoclonal Antibody (Catalog # MAB5526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for JunD at approximately 40 kDa and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JunD

JunD is a member of the Jun family of nuclear transcription factors. It dimerizes with other Jun or Fos family members to form the AP1 transcription factor. Unlike c‑Jun or JunB, JunD antagonizes Ras transformation and accumulates as cells enter quiescence. JunD is a 347 amino acid (aa), 39 kDa protein (JunD-FL) that has a 303 aa, 34 kDa isoform ( delta JunD) which begins at aa 44. Over the sequence used as an immunogen, human JunD shares 72% aa identity with mouse and rat JunD.

Long Name
JunD Proto-oncogene
Entrez Gene IDs
3727 (Human); 16478 (Mouse); 24518 (Rat)
Alternate Names
activator protein 1; AP-1; jun D proto-oncogene; JunD; JunD-FL isoform; transcription factor jun-D

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Citations for Human/Mouse JunD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An integrated multi-omics approach to identify regulatory mechanisms in cancer metastatic processes
    Authors: S Ghaffari, C Hanson, RE Schmidt, KJ Bouchonvil, SM Offer, S Sinha
    Genome Biology, 2021;22(1):19.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.
    Authors: Kashiwada M, Cassel SL, Colgan JD
    EMBO J., 2011;30(10):2071-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot


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