Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine, Canine, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 355521
View all Myocardin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Myocardin
Met97-Leu290
Accession # Q8IZQ8

Specificity

Detects human and mouse Myocardin in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse Myocardin Antibody

Detection of Human Myocardin antibody by Western Blot.

Detection of Human Myocardin by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Myocardin by Western Blot

Detection of Mouse Myocardin by Western Blot

Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Myocardin by Western Blot

Detection of Mouse Myocardin by Western Blot

Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Myocardin by Western Blot

Detection of Mouse Myocardin by Western Blot

Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Myocardin by Western Blot

Detection of Mouse Myocardin by Western Blot

Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Myocardin by Western Blot

Detection of Myocardin by Western Blot

Differences in cell behavior between pig arterial smooth muscle cells (ApSMCs) and venous smooth muscle cells (VpSMCs). (A) The purity of VSMCs was demonstrated with anti-smooth muscle actin immunofluorescent staining, following the protocol described in the Materials and Methods. Scale bars = 200 microns. (B,C) Cell proliferation under the growth medium (GM) or in response to PDGF-BB stimulation was measured following the protocol described in the Materials and Methods. Different letters (a, b) indicated significant difference between two groups. (D) Cell migration assays were performed via scratch assays in serum-free (SF) or 10% FBS containing GM. (E) Migrated cells in scratched areas were counted. Scale bars = 200 microns. (F) Cells were induced to differentiate with a differentiation medium (DM), and 48 h before collecting cell lysates, PDGF-BB was applied in the indicated wells. (G,H) The densitometry of each band was obtained using Image J (NIH version 1.53K), and ratio values were obtained by interested protein bands/loading control bands. (n = 6 for calponin; n = 5 for myocardin.) ****: p < 0.000; ***: p < 0.001; **: p < 0.01; *: p < 0.05; ns: no significant differences were found using a Student’s t-test for two-group comparisons and an ANOVA test for multiple group comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40243809), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Myocardin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB4028 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myocardin

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

Alternate Names

BSAC2A, MYCD, MYOCD, Srfcp

Entrez Gene IDs

93649 (Human); 214384 (Mouse); 246297 (Rat)

Gene Symbol

MYOCD

UniProt

Q8IZQ8

Additional Myocardin Products

Product Documents for Human/Mouse Myocardin Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Myocardin Antibody

For research use only

Citations for Human/Mouse Myocardin Antibody

Customer Reviews for Human/Mouse Myocardin Antibody (1)

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  • Human/Mouse Myocardin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: C2C12 mouse myoblast cell line
    Species: Mouse
    Verified Customer | Posted 09/01/2021
    Human/Mouse Myocardin Antibody MAB4028

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