Human/Mouse Myocardin Antibody

(8 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Myocardin in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 355521
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Myocardin
    Met97-Leu290
    Accession # Q8IZQ8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Myocardin

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

  • Entrez Gene IDs:
    93649 (Human); 214384 (Mouse); 246297 (Rat)
  • Alternate Names:
    BSAC2A; MYCD; Myocardin; MYOCD; Srfcp
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation
    J Am Heart Assoc, 2016;5(12):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
    Authors: Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R
    J Biol Chem, 2014;289(28):19585-98.
    Species: Canine
    Sample Type: Cell Lysates
    Application: WB
  3. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
    Authors: Yao Y, Chen Z, Norris E, Strickland S
    Nat Commun, 2014;5(0):3413.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
    Authors: Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J
    Circ Res, 2013;113(7):902-14.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
    Authors: Abdalla M, Goc A, Segar L, Somanath P
    J Biol Chem, 2013;288(46):33483-93.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
    Authors: Lu, Tzong-Sh, Lim, Kenneth, Molostvov, Guerman, Yang, Yun-Chun, Yiao, Szu-Yu, Zehnder, Daniel, Hsiao, Li-Li
    Cardiovasc Res, 2012;96(3):524-32.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
    Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
    J. Biol. Chem., 2011;286(32):28097-110.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
    Authors: Pfisterer, Larissa, Feldner, Anja, Hecker, Markus, Korff, Thomas
    Cardiovasc Res, 2011;96(1):120-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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