Human/Mouse Myocardin Antibody

Catalog # Availability Size / Price Qty
MAB4028
MAB4028-SP

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Detection of Human Myocardin by Western Blot.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse Myocardin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Myocardin in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 355521
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Myocardin
Met97-Leu290
Accession # Q8IZQ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Myocardin antibody by Western Blot. View Larger

Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Myocardin by Western Blot View Larger

Detection of Mouse Myocardin by Western Blot Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Myocardin by Western Blot View Larger

Detection of Mouse Myocardin by Western Blot Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Myocardin by Western Blot View Larger

Detection of Mouse Myocardin by Western Blot Laminin-111 but not laminin-alpha 4 blocking antibody affects pericyte differentiation. (a) Immunoblots show that laminin-111 blockage (Ln Ab) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14b. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=5–6). (b) Immunoblots show that laminin-alpha 4 blockage (Anti-Ln alpha 4) does not change the expression of PDGFR beta, SMA, SM22-alpha, or myocardinin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14c. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=3). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Myocardin by Western Blot View Larger

Detection of Mouse Myocardin by Western Blot Astrocytic laminin mediates pericyte differentiation via integrin alpha 2. (a) Immunoblots show that integrin alpha 2 blockage (ITGA2) but not integrin beta 1 blockage significantly increases the expression of PDGFR beta, SMA, and SM22-alpha, but not myocardin in pericytes. Full blots of these proteins are shown in Supplementary Figure 14d. Rabbit IgG treated cells were used as a Ctr. All bands were normalized to actin (n=6). (b) Schematic diagram of shRNA designed to target ITGA2 mRNA. (c) Immunoblot analysis shows that all three ITGA2-specific shRNAs (#1–3) dramatically reduce ITGA2 at protein level and ITGA2-specific shRNA-3 (#1) is the most efficient one. Full blots of ITGA2 and actin are shown in Supplementary Figure 14e. Scramble shRNA was used as a Ctr. (d) Immunoblot analysis shows that transduction of pericytes with lenti-shRNA-1 (#1) significantly enhances the expression of PDGFR beta, SMA, and SM22-alpha, but does not affect myocardin level. Full blots of these proteins are shown in Supplementary Figure 14f. Scramble shRNA was used as a Ctr. All bands were normalized to actin (n=4–5). Data are shown as mean ± sd. *p<0.05 versus the Ctrs by student’s t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24583950), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myocardin

Myocardin (MYOCD) is a transcriptional co-activator necessary for differentiation of smooth muscle cells. MYOCD functions by binding the transcription factor Serum Response Factor (SRF) and stimulating smooth muscle cell-specific gene expression.

Entrez Gene IDs
93649 (Human); 214384 (Mouse); 246297 (Rat)
Alternate Names
BSAC2A; MYCD; Myocardin; MYOCD; Srfcp

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Citations for Human/Mouse Myocardin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Alpha-enolase regulates the malignant phenotype of pulmonary artery smooth muscle cells via the AMPK-Akt pathway
    Authors: J Dai, Q Zhou, J Chen, ML Rexius-Hal, J Rehman, G Zhou
    Nat Commun, 2018-09-21;9(1):3850.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC, Western Blot
  2. Downregulation of Insulin Receptor Substrate-1 During Hyperglycemia Induces Vascular Smooth Muscle Cell Dedifferentiation
    Authors: Gang Xi
    J. Biol. Chem, 2016-12-21;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. miR-17/20 Controls Prolyl Hydroxylase 2 (PHD2)/Hypoxia-Inducible Factor 1 (HIF1) to Regulate Pulmonary Artery Smooth Muscle Cell Proliferation.
    Authors: Tianji Chen, Qiyuan Zhou, Haiyang Tang, Melike Bozkanat, Jason X-J Yuan, J Usha Raj, Guofei Zhou
    Journal of the American Heart Association, 2016-12-05;0(0):2047-9980.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Co-activation of nuclear factor-kappaB and myocardin/serum response factor conveys the hypertrophy signal of high insulin levels in cardiac myoblasts.
    Authors: Madonna R, Geng Y, Bolli R, Rokosh G, Ferdinandy P, Patterson C, De Caterina R
    J Biol Chem, 2014-05-22;289(28):19585-98.
    Species: Canine, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  5. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
    Authors: Yao Y, Chen Z, Norris E, Strickland S
    Nat Commun, 2014-03-03;5(0):3413.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Akt1 mediates alpha-smooth muscle actin expression and myofibroblast differentiation via myocardin and serum response factor.
    Authors: Abdalla M, Goc A, Segar L, Somanath P
    J Biol Chem, 2013-10-08;288(46):33483-93.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Transplantation of mesenchymal cells rejuvenated by the overexpression of telomerase and myocardin promotes revascularization and tissue repair in a murine model of hindlimb ischemia.
    Authors: Madonna R, Taylor D, Geng Y, De Caterina R, Shelat H, Perin E, Willerson J
    Circ Res, 2013-06-18;113(7):902-14.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Induction of intracellular heat-shock protein 72 prevents the development of vascular smooth muscle cell calcification.
    Authors: Lu, Tzong-Sh, Lim, Kenneth, Molostvov, Guerman, Yang, Yun-Chun, Yiao, Szu-Yu, Zehnder, Daniel, Hsiao, Li-Li
    Cardiovasc Res, 2012-08-29;96(3):524-32.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Hypertension impairs myocardin function: a novel mechanism facilitating arterial remodelling.
    Authors: Pfisterer, Larissa, Feldner, Anja, Hecker, Markus, Korff, Thomas
    Cardiovasc Res, 2012-07-26;96(1):120-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Down-regulation of Kruppel-like Factor-4 (KLF4) by MicroRNA-143/145 Is Critical for Modulation of Vascular Smooth Muscle Cell Phenotype by Transforming Growth Factor-{beta} and Bone Morphogenetic Protein 4.
    Authors: Davis-Dusenbery BN, Chan MC, Reno KE, Weisman AS, Layne MD, Lagna G, Hata A
    J. Biol. Chem., 2011-06-13;286(32):28097-110.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse Myocardin Antibody
By Anonymous on 09/01/2021
Application: WB Sample Tested: C2C12 mouse myoblast cell line Species: Mouse