Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody
Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody Summary
Val332-Lys558
Accession # Q06219
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Nurr1/NGFI‑B beta /NR4A2 in HeLa Human Cell Line. Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Nurr1/NGFI‑B beta /NR4A2 in Mouse Brain (Hippocampus). Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nurr1/NGFI-B beta/NR4A2
Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.
Product Datasheets
Citations for Human/Mouse Nurr1/NGFI-B beta /NR4A2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Inhibition of CSPG receptor PTPsigma promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke
Authors: F Luo, J Wang, Z Zhang, Z You, A Bedolla, F Okwubido-W, LF Huang, J Silver, Y Luo
Oncogene, 2022;40(4):111137.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Regulation of prefrontal patterning and connectivity by retinoic acid
Authors: M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan
Nature, 2021;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Striatal Nurr1 Facilitates the Dyskinetic State and Exacerbates Levodopa-Induced Dyskinesia in a Rat Model of Parkinson's Disease
Authors: RC Sellnow, K Steece-Col, F Altwal, IM Sandoval, JH Kordower, TJ Collier, CE Sortwell, AR West, FP Manfredsso
J. Neurosci., 2020;40(18):3675-3691.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex
Authors: Y Arai, AW Cwetsch, E Coppola, S Cipriani, H Nishihara, H Kanki, Y Saillour, B Freret-Hod, A Dutriaux, N Okada, H Okano, C Dehay, J Nardelli, P Gressens, T Shimogori, G D'Onofrio, A Pierani
Cell Rep, 2019;29(3):645-658.e5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Genome Stability by DNA polymerase ? in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development
Authors: K Onishi, A Uyeda, M Shida, T Hirayama, T Yagi, N Yamamoto, N Sugo
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Extracortical origin of some murine subplate cell populations.
Authors: Pedraza M, Hoerder-Suabedissen A, Albert-Maestro M, Molnar Z, De Carlos J
Proc Natl Acad Sci U S A, 2014;111(23):8613-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Deficiency of the NR4A neuron-derived orphan receptor-1 attenuates neointima formation after vascular injury.
Authors: Nomiyama T, Zhao Y, Gizard F, Findeisen HM, Heywood EB, Jones KL, Conneely OM, Bruemmer D
Circulation, 2009;119(4):577-86.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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