Olig1 in Human Glioma.
Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Olig1 by Western Blot.
Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Olig1 and Olig2, which have been implicated in oligodendrogenesis, are
expressed in the region of the ventral ventricular zone of late
embryonic spinal cord where oligodendrocyte progenitors appear. Olig3
is transiently expressed in different types of progenitors of embryonic
central nervous system and then disappears in the course of
development. In adult, expression of Olig3 is primarily detected in
Oligodendrocyte Lineage Gene 1
Entrez Gene IDs:
BHLHB6; bHLHe21; Class B basic helix-loop-helix protein 6; Class E basic helix-loop-helix protein 21; helix-loop-helix protein, class B, 6; Olig1; oligo1; oligodendrocyte lineage transcription factor 1; oligodendrocyte transcription factor 1; oligodendrocyte-specific bHLH transcription factor 1
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