Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Camelid - Vicugna pacos (Alpaca), Canine, Transgenic Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 97505
View all Orexin A/Hypocretin-1 Primary Antibodies »

Product Specifications

Immunogen

KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612

Specificity

Detects human and mouse Orexin A/Hypocretin‑1. In dot blots, no cross-reactivity with recombinant human Orexin B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Orexin A/Hypocretin‑1 Antibody

Orexin A/Hypocretin‑1 in Mouse Hypothalamus.

Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Monoclonal Antibody (Catalog # MAB763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse Orexin A/Hypocretin‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Hypothalamus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Orexin A/Hypocretin-1

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).

References

  1. Sakurai, T. et al. (1998) Cell 92:573.

Alternate Names

HCRT, Hcrt1, Hypocretin-1, Hypocretin1

Entrez Gene IDs

3060 (Human); 25723 (Rat)

Gene Symbol

HCRT

UniProt

O43612

Additional Orexin A/Hypocretin-1 Products

Product Documents for Human/Mouse Orexin A/Hypocretin‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Orexin A/Hypocretin‑1 Antibody

For research use only

Citations for Human/Mouse Orexin A/Hypocretin‑1 Antibody

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Protocols

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