Human/Mouse Orexin A/Hypocretin-1 Antibody

Catalog # Availability Size / Price Qty
MAB763-SP
MAB763-500
MAB763-100

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Orexin A/Hypocretin‑1 in Mouse Hypothalamus.
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Citations (11)
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Human/Mouse Orexin A/Hypocretin-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Orexin A/Hypocretin‑1. In dot blots, no cross-reactivity with recombinant human Orexin B is observed.
Source
Monoclonal Mouse IgG1 Clone # 97505
Purification
Protein A or G purified from ascites
Immunogen
KLH-coupled human Orexin A/Hypocretin‑1 synthetic peptide
Accession # O43612
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Hypothalamus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Orexin A/Hypocretin‑1 in Mouse Hypothalamus. Orexin A/Hypocretin‑1 was detected in immersion fixed paraffin-embedded sections of Mouse Hypothalamus using Mouse Anti-Human/Mouse Orexin A/Hypocretin‑1 Monoclonal Antibody (Catalog # MAB763) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin A/Hypocretin-1

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein. Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism. The synthetic peptide immunogen corresponds to amino acid 34 - 66 of the human Orexin precursor (accession #O43612) (1).

References
  1. Sakurai, T. et al. (1998) Cell 92:573.
Entrez Gene IDs
3060 (Human); 25723 (Rat)
Alternate Names
HCRT; Hcrt1; hypocretin (orexin) neuropeptide precursor; Hypocretin1; Hypocretin-1; NRCLP1; Orexin A; orexin; OXHcrt; PPORX; PPOXHypocretin

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Citations for Human/Mouse Orexin A/Hypocretin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Potential role of orexin A binding the receptor 1 for orexins in normal and cryptorchid dogs
    Authors: G Liguori, C Squillacio, L Assisi, A Pelagalli, A Vittoria, A Costagliol, N Mirabella
    BMC Vet. Res., 2018-02-27;14(1):55.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  2. Vasopressin-secreting neurons derived from human embryonic stem cells through specific induction of dorsal hypothalamic progenitors
    Authors: K Ogawa, H Suga, C Ozone, M Sakakibara, T Yamada, M Kano, K Mitsumoto, T Kasai, Y Kodani, H Nagasaki, N Yamamoto, D Hagiwara, M Goto, R Banno, Y Sugimura, H Arima
    Sci Rep, 2018-02-26;8(1):3615.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)
    Authors: G Liguori, C Squillacio, L Assisi, N Mirabella, E Langella, A Costagliol, A Vittoria
    Reprod. Domest. Anim, 2017-02-08;0(0):.
    Species: Alpaca
    Sample Types: Whole Tissue
    Applications: IHC
  4. Orexin/hypocretin system modulates amygdala-dependent threat learning through the locus coeruleus.
    Authors: Sears, Robert M, Fink, Ann E, Wigestrand, Mattis B, Farb, Claudia, de Lecea, Luis, Ledoux, Joseph E
    Proc Natl Acad Sci U S A, 2013-11-25;110(50):20260-5.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Endoplasmic reticulum stress in wake-active neurons progresses with aging.
    Authors: Naidoo N, Zhu J, Zhu Y, Fenik P, Lian J, Galante R, Veasey S
    Aging Cell, 2011-04-12;10(4):640-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. SIRT1 regulation of wakefulness and senescence-like phenotype in wake neurons.
    Authors: Panossian L, Fenik P, Zhu Y
    J. Neurosci., 2011-03-16;31(11):4025-36.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Hypocretins regulate the anxiogenic-like effects of nicotine and induce reinstatement of nicotine-seeking behavior.
    Authors: Plaza-Zabala A, Martin-Garcia E, de Lecea L, Maldonado R, Berrendero F
    J. Neurosci., 2010-02-10;30(6):2300-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Genetic mapping of Foxb1-cell lineage shows migration from caudal diencephalon to telencephalon and lateral hypothalamus.
    Authors: Zhao T, Szabo N, Ma J, Luo L, Zhou X, Alvarez-Bolado G
    Eur. J. Neurosci., 2008-11-01;28(10):1941-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. An essential role for orexins in emergence from general anesthesia.
    Authors: Kelz MB, Sun Y, Chen J, Cheng Meng Q, Moore JT, Veasey SC, Dixon S, Thornton M, Funato H, Yanagisawa M
    Proc. Natl. Acad. Sci. U.S.A., 2008-01-14;105(4):1309-14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. REM sleep changes in rats induced by siRNA-mediated orexin knockdown.
    Authors: Chen L, Thakkar MM, Winston S, Bolortuya Y, Basheer R, McCarley RW
    Eur. J. Neurosci., 2006-10-01;24(7):2039-48.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Involvement of the lateral hypothalamic peptide orexin in morphine dependence and withdrawal.
    Authors: Georgescu D, Zachariou V, Barrot M, Mieda M, Willie JT, Eisch AJ, Yanagisawa M, Nestler EJ, DiLeone RJ
    J. Neurosci., 2003-04-15;23(8):3106-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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