Detection of Human/Mouse Park7/DJ‑1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Park7/DJ‑1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Park7/DJ‑1 in HeLa Human Cell Line.
Park7/DJ‑1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human Park7/DJ‑1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3668) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Park7/DJ‑1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the
ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function
as protein chaperones, catalases, proteases and kinases. Park7 is
widely expressed in the brain as well as in peripheral tissues. It
exists as a homodimer that can be localized in the cytoplasm, nucleus
and mitochondria. Park7 is a redox-sensitive protein that has been
ascribed various functions including that as a redox sensor and
antioxidant protein. Mutations in Park7 are associated with a small
percentage of hereditary early onset Parkinson’s disease. Human and
mouse Park7 share 92% amino acid sequence identity.
Parkinson Disease 7
Entrez Gene IDs:
11315 (Human); 57320 (Mouse); 117287 (Rat)
DJ1; DJ-1; DJ1FLJ34360; EC 3.4; FLJ27376; FLJ92274; Oncogene DJ1; Park7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; protein DJ-1
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