Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB18251-SP
MAB18251-100
Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.
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Product Details
Citations (1)
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Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1088B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human ERK1 T202/Y204  site
Accession # P27361
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot. View Larger

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB18251) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) at approximately 44 kDa and Phospho-ERK2 (T185/Y187) at approximately 42 (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot. View Larger

Detection of Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF‑BB (Catalog # 220-BB) for 5 minutes. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB18251) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) at approximately 44 kDa and Phospho-ERK2 (T185/Y187) at approximately 42 (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. View Larger

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with PMA and Ionomycin, loaded at 0.2 mg/mL. Specific bands were detected for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 46-49 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB18251). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name
Extracellular Signal-regulated Kinase 1
Entrez Gene IDs
5595 (Human); 26417 (Mouse); 50689 (Rat)
Alternate Names
EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24

Product Datasheets

Citation for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tumor associated macrophages induce epithelial to mesenchymal transition via the EGFR/ERK1/2 pathway in head and neck squamous cell carcinoma
    Authors: L Gao, W Zhang, WQ Zhong, ZJ Liu, HM Li, ZL Yu, YF Zhao
    Oncol. Rep., 2018;40(5):2558-2572.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Reviews for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

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Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody
By Anonymous on 07/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human