Detection of Human and Mouse PVRIG by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, CEM human T-lymphoblastoid cell line, and TK‑1 mouse T cell lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PVRIG at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PVRIG in MOLT‑4 Human Cell Line.
PVRIG was detected in immersion fixed MOLT‑4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human/Mouse PVRIG Monoclonal Antibody (Catalog # MAB9365) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human PVRIG (poliovirus
receptor related immunoglobulin domain-containing protein), also known as CD112
receptor (CD112R), is an approximately 34 kDa single
transmembrane protein in the poliovirus receptor-like
protein (PVR) family (1). It is composed of a single extracellular
IgV domain, one transmembrane domain, and a long intracellular domain. The
intracellular domain contains two tyrosine residues, one within an ITIM-like
motif that is a potential docking site for phosphatases (1). The extracellular domain sequence
of human and mouse PVRIG have approximately 65% similarity. The human PVRIG
gene is preferentially expressed in lymphocytes, such as T cells and NK cells,
but not in monocyte derived dendritic cells (1). PVRIG functions as a cell
surface receptor for Nectin-2/CD112, a cell surface protein that is widely
expressed on antigen-presenting cells and tumor cells. Disrupting the
PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is
a novel checkpoint for human T cells (1).
Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.
Poliovirus Receptor Related Immunoglobulin Domain Containing
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