Human/Mouse R‑Spondin 1 Antibody

Name: Human/Mouse R‑Spondin 1 Antibody
Brand: R&D Systems
SKU: MAB4658
Price: 459 USD
Availability: InStock

R&D Systems | Catalog # MAB4658

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Citations (3)

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 422407

View all R-Spondin 1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human R-Spondin 1
Met1-Gly182
Accession # NP_775911

Specificity

Detects human and mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) R-Spondin 2 or rhR-Spondin 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human/Mouse R‑Spondin 1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human R-Spondin 1 (Catalog # 4645-RS)
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: R-Spondin 1

RSPO-1 (roof plate-specific spondin; also R-spondin1 and cristin3) is a 39 kDa, heparin-binding member of the Cristin/R-spondin family of proteins. These molecules apparently are either retained intracellularly or secreted, where they activate the frizzled-LRP receptor pathway. Mature human RSPO-1 is 27 kDa and 243 amino acids (aa) in length. It contains a cysteine-rich furin repeat domain followed by a type I TSP domain. This form is apparently retained intracellularly. A presumably glycosylated, 30 kDa, 193 aa C-terminally truncated form is secreted and interacts with heparin. The secreted form of human RSPO-1 is 93% aa identical to secreted mouse RSPO-1.