Human/Mouse/Rat CART Antibody

Catalog #: AF163
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF163
AF163-SP

Request a Quote Bulk Orders

2 Images

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat CART Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human CART in direct ELISAs and Western blots. Also recognizes a truncated recombinant human CART containing amino acids 69‑116.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CART
Gln28-Leu116
Accession # Q16568

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CART

Immunohistochemistry

1-15 µg/mL

Immersion fixed paraffin-embedded sections of human brain (hippocampus) tissue and perfusion fixed frozen sections of mouse brain tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

CART in Human Brain Hippocampus Tissue. CART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

View Larger

CART in Mouse Brain Tissue. CART was detected in perfusion fixed frozen sections of mouse brain tissue using Goat Anti-Human/Mouse/Rat CART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF163) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to neurons. Staining was performed using our Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CART

CART, also known as cocaine and amphetamine-regulated transcript, is a 10 kDa secreted polypeptide produced by neuronal cell types in a variety of locations. In human, the molecule is synthesized as a 116 amino acid (aa) precursor with a 27 aa signal sequence an 89 aa mature segment. There are six C-terminal cysteines that form three intrachain disulfide bonds, and the potential exists for both sulfation and phosphorylation in the N-terminal region. There are numerous monobasic and dibasic sites for enzyme cleavage, and multiple, presumably proteolytically-generated short forms are known to exist. These include a 5K, 48 aa peptide (CART 42‑89), and a 4.3K, 40 aa peptide (CART 49-89). Human CART is 98% aa identical to both moyuse and rat CART (1-89). Although CART suppresses feeding and increases pain tolerance, there would appear to be differences between the various forms of CART on other functions. In addition, anatomical location is associated with differing lengths of CART. For example, 89 aa and 79 aa forms are found in the adrenal, while 48 aa and 44 aa forms are found in the hypothalamus.

References

Douglass, J. & S. Daoud (1996) Gene 169:241.
Douglass, J. et al. (1995) J. Neurosci. 15:247.
Adams, L.D. et al. (1999) Brain Res. 848:137.
Thim, L. et al. (1999) Proc. Natl. Acad. Sci. USA 96:2722.
Bannon, A.W. et al. (2001) J. Pharmacol. Exp. Ther. 299:1021.
Kuhar, M.J. & L.L. Yoho (1999) Synapse 33:163.
Thim, L. et al. (1998) FEBS Lett. 428:263.

Long Name

Cocaine- and Amphetamine-regulated Transcript Protein

Entrez Gene IDs

9607 (Human)

Alternate Names

CART prepropeptide; CART; CARTcocaine and amphetamine regulated transcript; CARTPT; cocaine- and amphetamine-regulated transcript protein

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat CART Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

MCT2 expression and lactate influx in anorexigenic and orexigenic neurons of the arcuate nucleus.
Authors: Cortes-Campos C, Elizondo R, Carril C, Martinez F, Boric K, Nualart F, Garcia-Robles M
PLoS ONE, 2013;8(4):e62532.
Species: Rat
Sample Types: Whole Cells
Applications: ICC