Human/Mouse/Rat Catalase Antibody
Human/Mouse/Rat Catalase Antibody Summary
Met1-Leu527
Accession # P04040
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse/Rat Catalase by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
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Detection of Human Catalase by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Catalase in HL‑60 Human Cell Line. Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Western Blot Shows Human Catalase Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Catalase
Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.
Product Datasheets
Citations for Human/Mouse/Rat Catalase Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif
Authors: MA Farelo, D Korrou-Kar, KF Brooks, TA Russell, K Maringer, PU Mayerhofer
Viruses, 2022;14(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Olive oil with high polyphenolic content induces both beneficial and harmful alterations on rat redox status depending on the tissue
Authors: P Kouka, F Tekos, Z Papoutsaki, P Stathopoul, M Halabalaki, M Tsantarlio, I Zervos, C Nepka, J Liesivuori, VN Rakitskii, A Tsatsakis, AS Veskoukis, D Kouretas
Toxicol Rep, 2020;7(0):421-432.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases
Authors: A Yammine, A Zarrouk, T Nury, A Vejux, N Latruffe, D Vervandier, M Samadi, JJ Mackrill, H Greige-Ger, L Auezova, G Lizard
Cells, 2020;9(11):.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot -
Mitochondrial function in liver cells is resistant to perturbations in NAD+ salvage capacity
Authors: M Dall, SAJ Trammell, M Asping, AS Hassing, M Agerholm, SG Vienberg, MP Gillum, S Larsen, JT Treebak
J. Biol. Chem., 2019;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A critical role of autophagy in antileukemia/lymphoma effects of APO866, an inhibitor of NAD biosynthesis.
Authors: Ginet, Vanessa, Puyal, Julien, Rummel, Coralie, Aubry, Dominiqu, Breton, Caroline, Cloux, Anne-Jul, Majjigapu, Somi R, Sordat, Bernard, Vogel, Pierre, Bruzzone, Santina, Nencioni, Alessio, Duchosal, Michel A, Nahimana, Aimable
Autophagy, 2014;10(4):603-17.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4
Authors: Kwon IK, Wang R, Thangaraju M
Oncogene, 2010;29(0):3423.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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Reviews for Human/Mouse/Rat Catalase Antibody
Average Rating: 4.3 (Based on 4 Reviews)
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we overexpressed catalase contained plasmid in HEK293 cells and used 1:1000 dilution to detect this antibody.
