Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Catalase Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Catalase
Met1-Leu527
Accession # P04040

Specificity

Detects human, mouse and rat Catalase in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Catalase Antibody

Detection of Human/Mouse/Rat Catalase antibody by Western Blot.

Detection of Human/Mouse/Rat Catalase by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Detection of Human Catalase by Simple WesternTM.

Simple Western lane view shows lysates of Exosome Standards (HEK293) (NBP3-11684) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Catalase at approximately 61 kDa (as indicated) using 10 µg/ml of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Human Catalase antibody by Simple WesternTM.

Detection of Human Catalase by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Catalase antibody in HL-60 Human Cell Line by Immunocytochemistry (ICC).

Catalase in HL‑60 Human Cell Line.

Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Western Blot Shows Human Catalase Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Catalase Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Catalase by Western Blot

Detection of Catalase by Western Blot

Representative western blots showing the effects of grape pomace extract (GPE) on the expression of (A) gamma-glutamylcysteine synthetase (GCS), (B) catalase (CAT), (C) superoxide dismutase (SOD) and (D) heme oxygenase 1 (HO-1) in EA.hy926 endothelial cells. The results of densitometric quantification for all enzymes are also shown. The cells were incubated with GPE at 0.068 and 0.250 µg/ml for 3, 6, 12, 18 and 24 h. The expression of GAPDH was used as a loading control for normalization. *P<0.05, statistically significant difference compared to the control (untreated cells). The results are presented as the means ± SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26082074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Catalase by Western Blot

Detection of Catalase by Western Blot

Representative western blots showing the effects of grape pomace extract (GPE) on the expression of (A) gamma-glutamylcysteine synthetase (GCS), (B) catalase (CAT), (C) superoxide dismutase (SOD) and (D) heme oxygenase 1 (HO-1) in C2C12 muscle cells. The results of densitometric quantification for all enzymes are also shown. The cells were incubated with GPE at 2.5 and 10 µg/ml for 3, 6, 12, 18 and 24 h. The expression of GAPDH was used as a loading control for normalization. *P<0.05, statistically significant difference compared to the control (untreated cells). The results are presented as the mean ± SEM. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26082074), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Catalase by Western Blot

Detection of Catalase by Western Blot

Effect of argan oil, olive oil or colza oil treatment on brain expressions of peroxisomal proteins, CAT (A), ACOX1 (B), ABCD1 (C), ABCD2 (D) and the heatmap for all protein expression (E). Male C57BL/6 mice received for 28 days a standard diet (control (CTRL)), a diet enriched with 6% (w/w) AO, OO, or CO, and intravenous injection of LPS (100 µg) four hours antemortem. Brain homogenates were separated in PAGE-SDS electrophoresis and subjected to immunoblotting as described in Section 4. Band intensities were analyzed by densitometry and standardized to beta -actine expression level. Tables represent the standardized densitometric analysis obtained after signal intensity quantification of different proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35455460), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Catalase by Western Blot

Detection of Catalase by Western Blot

Effect of argan oil, olive oil or colza oil treatment on liver expressions of peroxisomal proteins, CAT (A), ACOX1 (B), ABCD1 (C), ABCD2 (D) and the heatmap for all protein expression (E). Male C57BL/6 mice received for 28 days a standard diet (control (CTRL)), a diet enriched with 6% (w/w) AO, OO, or CO, and intravenous injection of LPS (100 µg) four hours antemortem. Liver homogenates were prepared as described in Section 4. Band intensities were analyzed by densitometry and standardized to beta -actin expression level. Tables represent the standardized densitometric analysis obtained after signal intensity quantification of different proteins. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35455460), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Catalase Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed HL-60 human acute promyelocytic leukemia cell line

Knockout Validated

Catalase is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Catalase knockout HeLa cell line.

Simple Western

5-10 µg/mL
Sample: Exosome Standards (HEK293) (Catalog # NBP3-11684), Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 7 reviews rated 4.6 using AF3398 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Catalase

Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.

Alternate Names

Cas1, CAT, Cs-1

Entrez Gene IDs

847 (Human); 12359 (Mouse); 24248 (Rat)

Gene Symbol

CAT

UniProt

P04040

Additional Catalase Products

Product Documents for Human/Mouse/Rat Catalase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat Catalase Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Oxidative Stress

Citations for Human/Mouse/Rat Catalase Antibody

Customer Reviews for Human/Mouse/Rat Catalase Antibody (7)

4.6 out of 5
7 Customer Ratings
5 Stars
71%
4 Stars
14%
3 Stars
14%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 7 reviews Showing All
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  • Human/Mouse/Rat Catalase Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/23/2021
    we overexpressed catalase contained plasmid in HEK293 cells and used 1:1000 dilution to detect this antibody.
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Nastaran Daneshgar
    Application: Western Blot
    Sample Tested: Kidney tissue and HK-2 human proximal tubule epithelial cell line
    Species: Mouse and Human
    Verified Customer | Posted 01/05/2021
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Maksim Baranov
    Application: Western Blot
    Sample Tested: Hek 293T cell lysate and RAW264.7 whole cell lysate
    Species: Human and Mouse
    Verified Customer | Posted 10/01/2019
    Restained with goat-anti-Catalase (1:50) - 2 nights 4C 2nd antibody Donkey-aa=nti-Goat-800 (1:5000) 30 min RT
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Maksim Baranov
    Application: Western Blot
    Sample Tested: HEK 293 cell line, HEK 293 cell lysate, Monocyte-derived dendritic cells, RAW264.7 whole cell lysate, Hek 293T cell lysate and Monocyte-derived immature dendritic cells
    Species: Human
    Verified Customer | Posted 10/01/2019
    The blot was restained with the goat-anti-Catalase AB (R&amp;D Systems, cat AF3398, Lot WVM0314101) - 1:50 - 2 days 4C
    The blot was restained with the goat-anti-Catalase AB (R&amp;D Systems, cat AF3398, Lot WVM0314101) - 1:50 - 2 days 4C (anti-FLAG-bands are from previous staining) 2nd AB - Donkey-anti-Goat-800 (1:5000) 30 min at RT The blot shows clear expression of Catalase by HEKs and human moDC (65kDa band)
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Maksim Baranov
    Application: Immunocytochemistry
    Sample Tested: Monocyte-derived immature dendritic cells
    Species: Human
    Verified Customer | Posted 10/01/2019
    Human moDCs stained with goat anti-Catalase antibody (AF3398) at 1:20 (10 ug/mL), rabbit anti-LAMP1 and mouse anti-EEA1.
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse/Rat Catalase Antibody AF3398
  • Human/Mouse/Rat Catalase Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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