Human/Mouse/Rat CDC25A Antibody

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Detection of Human/Mouse/Rat CDC25A by Western Blot.
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Product Details
Citations (2)
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Reviews (1)

Human/Mouse/Rat CDC25A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.
Monoclonal Mouse IgG2B Clone # 336445
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CDC25A
Accession # AAA58415
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat CDC25A antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat CDC25A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDC25A

Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.

  1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
  2. Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
  3. Hoffmann, I. et al. (1995) EMBO J. 13:4302.
  4. Sagata, N. (2002) Science 298:1905.
  5. Gautier, J. et al. (1991) Cell 67:197.
Long Name
Cell Division Cycle 25A
Entrez Gene IDs
993 (Human)
Alternate Names
CDC25A; CDC25A2; CDC25A2-CAG isoform; cell division cycle 25 homolog A (S. cerevisiae); cell division cycle 25 homolog A (S. pombe); cell division cycle 25A; Dual specificity phosphatase Cdc25A; EC; M-phase inducer phosphatase 1

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Citations for Human/Mouse/Rat CDC25A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
    Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.
    Authors: Dutta-Simmons J, Zhang Y, Gorgun G, Gatt M, Mani M, Hideshima T, Takada K, Carlson NE, Carrasco DE, Tai YT, Raje N, Letai AG, Anderson KC, Carrasco DR
    Blood, 2009;114(13):2699-708.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human/Mouse/Rat CDC25A Antibody
By Anonymous on 09/22/2021
Application: IHC Sample Tested: Colon cancer cell line Species: Human