Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 336445
View all CDC25A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDC25A
Glu2-Leu523
Accession # AAA58415

Specificity

Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat CDC25A Antibody

Detection of Human/Mouse/Rat CDC25A antibody by Western Blot.

Detection of Human/Mouse/Rat CDC25A by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat CDC25A Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB1648 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDC25A

Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.

References

  1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
  2. Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
  3. Hoffmann, I. et al. (1995) EMBO J. 13:4302.
  4. Sagata, N. (2002) Science 298:1905.
  5. Gautier, J. et al. (1991) Cell 67:197.

Long Name

Cell Division Cycle 25A

Alternate Names

CDC25A2, CDC25A2-CAG isoform, cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25 homolog A (S. pombe), cell division cycle 25A, Dual specificity phosphatase Cdc25A, EC 3.1.3.48, M-phase inducer phosphatase 1

Entrez Gene IDs

993 (Human)

Gene Symbol

CDC25A

UniProt

AAA58415

Additional CDC25A Products

Product Documents for Human/Mouse/Rat CDC25A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CDC25A Antibody

For research use only

Related Research Areas

Cell Cycle
Phosphatases and Regulators

Citations for Human/Mouse/Rat CDC25A Antibody

Customer Reviews for Human/Mouse/Rat CDC25A Antibody (1)

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  • Human/Mouse/Rat CDC25A Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon cancer cell line
    Species: Human
    Verified Customer | Posted 09/22/2021
    Human/Mouse/Rat CDC25A Antibody MAB1648

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Protocols

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