Human/Mouse/Rat FABP2/I-FABP Antibody

Catalog # Availability Size / Price Qty
MAB30782-100
MAB30782-SP
Detection  of Human, Mouse, and Rat FABP2/I‑FABP by Western Blot.
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Human/Mouse/Rat FABP2/I-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FABP2/I-FABP in direct ELISAs. Detects human, mouse, and rat FABP2/I-FABP in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 989821
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FABP2/I-FABP
Met1-Asp132
Accession # P12104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human, Mouse, and Rat FABP2/I‑FABP by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP2/I‑FABP by Western Blot. Western blot shows lysates of human small intestine tissue, mouse small intestine tissue, and rat small intestine tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP2/I‑FABP Monoclonal Antibody (Catalog # MAB30782) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP2/I‑FABP at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FABP2/I‑FABP  in Human Small Intestine. View Larger

FABP2/I‑FABP in Human Small Intestine. FABP2/I‑FABP was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human/Mouse/Rat FABP2/I‑FABP Monoclonal Antibody (Catalog # MAB30782) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP2/I-FABP

Intestinal Fatty Acid Binding Protein (FABP2), also known as I-FABP or gFABP is a member of the fatty acid binding protein family of intracellular proteins. FABP2 is only expressed in intestine, and it mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variation of FABP2 is implicated in obesity and Type II diabetes.

Long Name
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs
2169 (Human); 25598 (Rat)
Alternate Names
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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