GDF-8/Myostatin Antibody

R&D Systems | Catalog # AF788

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GDF-8/Myostatin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-8/Myostatin
Asp268-Ser376
Accession # O08689

Specificity

Detects human, mouse, and rat GDF-8/Myostatin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for GDF-8/Myostatin Antibody

Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin antibody by Western Blot.

Detection of Recombinant Human, Mouse, and Rat GDF-8/Myostatin by Western Blot.

Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF-8/Myostatin (788-G8), Recombinant Human/Mouse/Rat GDF-11/BMP-11 (1958-GD), Recombinant Human BMP-6 (507-BP), and Recombinant Mouse BMP-6 (6325-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for GDF-8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GDF-8/Myostatin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

GDF‑8/Myostatin in Mouse Embryo.

GDF-8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.

Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.

Recombinant Mouse GDF-8/Myostatin (788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF-8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.

Applications for GDF-8/Myostatin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube)

Western Blot

0.1 µg/mL
Sample: Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788-G8)

Neutralization

Measured by its ability to neutralize GDF‑8/Myostatin-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.6-3 µg/mL in the presence of 30 ng/mL Recombinant Mouse GDF‑8/Myostatin.

Reviewed Applications

Read 4 reviews rated 3.5 using AF788 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.

References

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
  3. McPherron, A.C. et al. (1997) Nature 387:83.
  4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
  5. Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.

Long Name

Growth Differentiation Factor 8

Alternate Names

GDF8, MSLHP, MSTN, Myostatin

Entrez Gene IDs

2660 (Human); 17700 (Mouse)

Gene Symbol

MSTN

UniProt

O08689

Additional GDF-8/Myostatin Products

Product Documents for GDF-8/Myostatin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for GDF-8/Myostatin Antibody

For research use only

Citations for GDF-8/Myostatin Antibody

Customer Reviews for GDF-8/Myostatin Antibody (4)

3.5 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
25%

Have you used GDF-8/Myostatin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 of 4 reviews Showing All
Filter By:
Clear Filters X
  • Human/Mouse/Rat GDF-8/Myostatin Antibody
    Name: Sampath Kumar Anandan
    Application: Western Blot
    Sample Tested: Skeletal muscle tissue
    Species: Avian and Mouse
    Verified Customer | Posted 04/15/2024
    The test samples consisted of skeletal muscle tissue homogenates derived from mouse and avian muscle tissues. The homogenisation buffer contained protease inhibitor cocktail. The positive control used was 1μg of R&D systems recombinant myostatin/GDF8 (#788-G8). I had loaded 20μg of total protein for each of the avian and mouse samples, along with1μg of R&D systems recombinant myostatin/GDF8 (#788-G8) into a 4-15% gel. The electrophoresis was performed with MES running buffer at 90V constant current, and the proteins were subsequently blotted onto a 0.2μm nitrocellulose membrane. To check for transfer efficiency and protein integrity, i had stained the blot with Ponceau S and found that the transfer was efficient and the proteins were not degraded. Subsequently, i destained the blot with TBS-T and deionised water, and blocked the blots with 5% milk in TBS-T. Prior to incubating the membrane in primary antibody, i had cut the membrane such that one section contained all the animal tissue samples and the other section contained the recombinant GDF-8/myostatin and the western standard. The cut sections were incubated separately with identical concentrations of primary and secondary antibodies - R&D systems goat polyclonal GDF8 antibody (AF788-SP) at 2 µg/mL concentration, and R&D system anti-goat secondary antibody (HAF017) at 1:4000 dilution. The recombinant GDF-8/Myostatin positive control and the western standards produced clear and robust signals. However, the sections containing the samples derived from animal tissues (avian and mouse) failed to produce any discernible signal. I had repeated the same experiment above three times with increasing concentrations of primary antibody - 0.1, 0.25 and 2 µg/mL. However, all three experiments yielded the same result - strong signal for the recombinant GDF-8/Myostatin, but no visible signal detected with the avian and mouse tissue samples.
    GDF-8/Myostatin Antibody AF788
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Name: Zachary Patinkin
    Application: Simple Western
    Sample Tested: Human Skeletal Muscle Lysate
    Species: Human
    Verified Customer | Posted 06/23/2015
    Simple Western. 0.2 ug/uL human skeletal muscle tissue lysate. Precursor observed ~51 kDa; mature form observed at ~29 kDa.
    GDF-8/Myostatin Antibody AF788
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23829672
    Species: Human
    Verified Customer | Posted 01/09/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23297411
    Species: Mouse
    Verified Customer | Posted 01/09/2015

There are no reviews that match your criteria.

Showing  1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies