Detects human, mouse, and rat GDF-8/Myostatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity is observed with recombinant human/mouse/rat GDF-11. In Western blots, no cross-reactivity with recombinant human BMP‑6 and recombinant mouse BMP-6 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse GDF-8/Myostatin Asp268-Ser376 Accession # O08689
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize GDF‑8/Myostatin-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.6-3 µg/mL in the presence of 30 ng/mL Recombinant Mouse GDF‑8/Myostatin.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of Recombinant Human, Mouse, and Rat GDF‑8/Myostatin by Western Blot.
Western blot shows 25 ng of Recombinant Human/Mouse/Rat GDF‑8/Myostatin (Catalog # 788‑G8), Recombinant Human/Mouse/Rat GDF‑11/BMP‑11 (Catalog # 1958‑GD), Recombinant Human BMP‑6 (Catalog # 507‑BP), and Recombinant Mouse BMP‑6 (Catalog # 6325‑BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GDF‑8/Myostatin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
GDF‑8/Myostatin in Mouse Embryo.
GDF‑8/Myostatin was detected in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube) using Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Hemoglobin Expression Induced by GDF‑8/Myostatin and Neutralization by Mouse GDF‑8/Myostatin Antibody.
Recombinant Mouse GDF‑8/Myostatin (Catalog # 788-G8) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF‑8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF‑8/Myostatin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF788). The ND50 is typically 0.6-3 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Growth Differentiation Factor 8 (GDF-8), also known as myostatin, is a member of the TGF-beta superfamily that is expressed specifically in developing and adult skeletal muscle. GDF-8 cDNA encodes a 376 amino acid (aa) prepropeptide with a 24 aa residue signal peptide, a 223 aa residue amino-terminal propeptide, and a 109 aa residue carboxy-terminal mature protein. Mature GDF-8 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members. Similar to the TGF-beta s, activins and BMP-11, GDF-8 also contains one extra pair of cysteine residues that is not found in other family members. The bioactive form of GDF-8 is a homodimer with an apparent molecular weight of approximately 25 kDa. GDF-8 is highly conserved across species. At the amino acid sequence level, mature human, mouse, rat and cow GDF-8 are 100% identical. Within the TGF-beta superfamily, GDF-8 is most closely related to BMP-11, a mammalian protein that acts as a dorsal mesoderm and neural inducer in Xenopus explants. The two proteins share 90% amino acid sequence identity within their mature chain. A targeted disruption of GDF-8 in mouse results in large mice with a widespread increase in skeletal muscle mass, indicating that GDF-8 is a negative regulator of skeletal muscle growth. A mutation in the bovine GDF-8 gene has been shown to be responsible for the double-muscled phenotype in cattle breeds such as Belgian Blue cattle that is characterized by an increase in muscle mass. GDF-8 has also been shown to inhibit preadipocyte differentiation to adipocytes. Mature GDF-8 binds to activin type II receptors and the binding is antagonized by the activin-binding protein, follistatin. R&D Systems recombinant GDF-8 preparations have been shown to act similarly to Activin A in both the Xenopus animal cap and the K562 assays.
Storm, E.E. et al. (1994) Nature 368:639.
Sharma, M. et al. (1999) J. Cell Physiol. 180:1.
McPherron, A.C. et al. (1997) Nature 387:83.
Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306.
Kim, H.S. et al. (2001) Biochem. Biophys. Res. Commun. 281:902.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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