Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody

Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Rat Glutathione Peroxidase 2/GPX2 by Western Blot

Expression of various ROS-related proteins, pro-inflammatory factors, and an osteoclast differentiation factor RANKL in tissue samples of all treated groups at 24 weeks after surgery.(A) Representative Western blot analysis of transferrin, catalase, SOD1, SOD2, SOD3, GPx1, GPx2, TTR, NF-kappa B, IL-1 beta and RANKL in different groups. GAPDH was used as internal control. (B) Densitometric quantification of Western blot analysis for all tested proteins normalized to GAPDH (n = 3 for each group). *, P < 0.05, for results compared to those of the sham group; #, P < 0.05, for results compared to those of the group receiving an artificial knee joint only. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30125327), licensed under a CC-BY license. Not internally tested by R&D Systems.