Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot.
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Product Details
Citations (2)
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Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse and rat GPX2 in Western blots.
Monoclonal Mouse IgG2B Clone # 496010
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Glutathione Peroxidase 2
Accession # P18283
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Glutathione Peroxidase 2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 2/GPX2

Glutathione Peroxidase 2 (GPX2), also known as Gastrointestinal Glutathione Peroxidase, is a member of the glutathione peroxidase antioxidant enzyme family. These proteins protect cell surfaces, extracellular fluid components, and enzymes from oxidative stress by reducing hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX2 is a cytoplasmic homotetramer of 22 kDa subunits, each containing a selenocysteine residue at the active site. GPX2 is primarily expressed in the liver and intestinal epithelial cells where it participates in the detoxification of dietary organic hydroperoxides. It is upregulated in a variety of carcinomas and functions as an anti-inflammatory and anti-apoptotic molecule. Over amino acids 41‑190, human GPX2 shares 94% aa sequence identity with mouse and rat GPX2.

Entrez Gene IDs
2877 (Human); 14776 (Mouse); 29326 (Rat)
Alternate Names
EC 1.11.1; EC; gastrointestinal glutathione peroxidase 2; Gastrointestinal glutathione peroxidase; GIGPX; GI-GPx; glutathione peroxidase 2 (gastrointestinal); Glutathione Peroxidase 2; Glutathione peroxidase-gastrointestinal; Glutathione peroxidase-related protein 2; GPRP; GPRP-2; GPX2; GPx-2; GPx-GI; GSHPx-2; GSHPX-GI

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Citations for Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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    Authors: RD Horniblow, P Pathak, DL Balacco, A Acharjee, E Lles, G Gkoutos, AD Beggs, C Tselepis
    The Journal of nutritional biochemistry, 2021;0(0):108929.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis
    Authors: KT Peng, MH Tsai, CW Lee, YC Chiang, PC Chen, CC Chen, CH Chang, HN Shih, PJ Chang
    PLoS ONE, 2018;13(8):e0202501.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot


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Reconstitution Buffers

Secondary Antibodies

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