Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody Summary
Accession # P18283
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat Glutathione Peroxidase 2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, Hepa 1-6 mouse hepatoma cell line, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 2 Monoclonal Antibody (Catalog # MAB5470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione Peroxidase 2/GPX2
Glutathione Peroxidase 2 (GPX2), also known as Gastrointestinal Glutathione Peroxidase, is a member of the glutathione peroxidase antioxidant enzyme family. These proteins protect cell surfaces, extracellular fluid components, and enzymes from oxidative stress by reducing hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX2 is a cytoplasmic homotetramer of 22 kDa subunits, each containing a selenocysteine residue at the active site. GPX2 is primarily expressed in the liver and intestinal epithelial cells where it participates in the detoxification of dietary organic hydroperoxides. It is upregulated in a variety of carcinomas and functions as an anti-inflammatory and anti-apoptotic molecule. Over amino acids 41‑190, human GPX2 shares 94% aa sequence identity with mouse and rat GPX2.
Citations for Human/Mouse/Rat Glutathione Peroxidase 2/GPX2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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IRON-MEDIATED EPIGENETIC ACTIVATION OF NRF2 TARGETS
Authors: RD Horniblow, P Pathak, DL Balacco, A Acharjee, E Lles, G Gkoutos, AD Beggs, C Tselepis
The Journal of nutritional biochemistry, 2021;0(0):108929.
Sample Types: Cell Lysates
Applications: Western Blot
Dysregulated expression of antioxidant enzymes in polyethylene particle-induced periprosthetic inflammation and osteolysis
Authors: KT Peng, MH Tsai, CW Lee, YC Chiang, PC Chen, CC Chen, CH Chang, HN Shih, PJ Chang
PLoS ONE, 2018;13(8):e0202501.
Sample Types: Tissue Homogenates
Applications: Western Blot
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