Human/Mouse/Rat IKK gamma Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat IKK gamma in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IKK gamma
    Met1-Glu419
    Accession # Q9Y6K9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse/Rat IKK gamma by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IKK gamma (1 ng) was included. Specific bands for IKK gamma were detected at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IKK gamma by Simple WesternTM. Simple Western lane view shows lysates of MDA‑MB‑468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for IKK gamma at approximately 47-56 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Immunocytochemistry
IKK gamma in MCF‑7 Human Cell Line. IKK gamma was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK gamma
I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.
  • Long Name:
    IkB Kinase gamma
  • Entrez Gene IDs:
    8517 (Human); 309295 (Rat)
  • Alternate Names:
    FIP-3IP2; FIP3NF-kappa-B essential modifier; FIP3P; I-kappa-B kinase subunit gamma; IkB kinase gamma subunit; IkB kinase subunit gamma; IkB kinase-associated protein 1; IKBKG; IKK gamma; IKKAP1; IKKG; IKK-gammaIPD2; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; Inhibitor of nuclear factor kappa-B kinase subunit gamma; IP; IP1; NEMO; NEMOAMCBX1; NFkappaB essential modulator; NF-kappa-B essential modulator
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