Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IKK gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IKK gamma
Met1-Glu419
Accession # Q9Y6K9

Specificity

Detects human, mouse, and rat IKK gamma in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat IKK gamma Antibody

Detection of Human/Mouse/Rat IKK? antibody by Western Blot.

Detection of Human/Mouse/Rat IKK gamma by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human IKK gamma (1 ng) was included. Specific bands for IKK gamma were detected at approximately 50 and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IKK? antibody by Simple WesternTM.

Detection of Human IKK gamma by Simple WesternTM.

Simple Western lane view shows lysates of MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for IKK gamma at approximately 47-56 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
IKK? antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

IKK gamma in MCF‑7 Human Cell Line.

IKK gamma was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat IKK gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2684) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat IKK gamma Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line

Simple Western

5 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: MDA-MB-468 human breast cancer cell line and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 2 using AF2684 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IKK gamma

I kappa B kinase gamma (IKK gamma ) is also known as NF-kappa B essential modulator (NEMO), IKBKG, and FIP3. The active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma (50 and 55 kDa), phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. The regulatory IKK gamma is required for activation of the IKK complex.

Long Name

IkB Kinase gamma

Alternate Names

FIP3P, IKBKG, IKKAP1, NEMO

Entrez Gene IDs

8517 (Human); 309295 (Rat)

Gene Symbol

IKBKG

UniProt

Q9Y6K9

Additional IKK gamma Products

Product Documents for Human/Mouse/Rat IKK gamma Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat IKK gamma Antibody

For research use only

Citations for Human/Mouse/Rat IKK gamma Antibody

Customer Reviews for Human/Mouse/Rat IKK gamma Antibody (1)

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  • Human/Mouse/Rat IKK gamma Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 06/06/2019
    Human/Mouse/Rat IKK gamma Antibody AF2684
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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FAQs

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