Human/Mouse/Rat Importin beta /KPNB1 Antibody

Catalog # Availability Size / Price Qty
MAB8209
MAB8209-SP
Detection of Human, Mouse, and Rat Importin  beta /KPNB1 by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat Importin beta /KPNB1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Importin beta /KPNB1 in ELISA and Western Blot. It detects mouse and rat Importin beta /KPNB1 in Western Blots.
Source
Monoclonal Mouse IgG2B Clone # 845208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Importin beta /KPNB1
Met1-Gly155
Accession # Q14974
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Importin  beta /KPNB1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Importin beta /KPNB1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin beta /KPNB1 Monoclonal Antibody (Catalog # MAB8209) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin beta /KPNB1 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Importin  beta /KPNB1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Importin beta /KPNB1 in HeLa Human Cell Line. Importin beta /KPNB1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin beta /KPNB1 Monoclonal Antibody (Catalog # MAB8209) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin beta/KPNB1

KPNB1 (Karyopherin subunit beta 1), also called Importin beta 1, NTF97 (nuclear transport factor, 97 kDa) or PTAC97 (pore targeting complex, 97 kDa), is a member of the Importin b family of proteins. It is ubiquitously expressed and plays many roles in the nucleus. Primarily, KPNB1 transports various complexes from cytoplasm into nucleus, either alone or with a KPNA adaptor. The 876 amino acid (aa) human KPNB1 contains an N-terminal IBB/Importin b domain (aa 21-101), and eight HEAT (solenoid) domains (aa 124-726). A 731 aa splice variant begins with Met146, while an 845 aa variant shows a 2 aa substitution for aa 702-734. Binding sites for adaptors and for its regulator, Ran-GTP, overlap within the HEAT domains. Human KPNB1 aa 1-155 shares 99% and 97% aa sequence identity with mouse and rat KPNB1, respectively.

Long Name
Karyopherin beta 1
Entrez Gene IDs
3837 (Human); 16211 (Mouse); 24917 (Rat)
Alternate Names
IMB1; Impnb; Importin 1; importin 90; Importin beta; importin beta-1 subunit; importin subunit beta-1; importin-90; IPO1; IPOB; karyopherin (importin) beta 1; Karyopherin subunit beta-1; KPNB1; MGC2155; MGC2156; MGC2157; NTF97; NTF97Pore targeting complex 97 kDa subunit; Nuclear factor p97; PTAC97

Product Datasheets

Citation for Human/Mouse/Rat Importin beta /KPNB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat Importin beta /KPNB1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human/Mouse/Rat Importin beta /KPNB1 Antibody
By Anonymous on 03/30/2022
Application: Western Blot Sample Tested: U266 human myeloma cell line Species: Human

1 ug/ml works excellent in the myeloma cell lysate.


Human/Mouse/Rat Importin beta /KPNB1 Antibody
By Anonymous on 02/06/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse