Detection of Human/Mouse/Rat Keap1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Keap1 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human Keap1 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Keap1 at approximately 75 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Keap1 Monoclonal Antibody (Catalog # MAB3024). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Kelch-like ECH-associated protein 1 (Keap1) is a 624 amino acid, 69 kDa protein which interacts with the transcription factor NF-E2-related factor 2 (Nrf2). Keap1 represses Nrf2 function by sequestering Nrf2 in the cytoplasm. Keap1 contains an N-terminal BTB domain and six C-terminal KELCH domains (aa 327‑611) that interact with Nrf2. Dissociation of the two proteins in response to redox-sensitive cell stress is followed by the translocation of Nrf2 to the nucleus and transcription of detoxifying/oxidative stress enzyme genes.
Kelch-like ECH-associated Protein 1
Entrez Gene IDs:
INrf2; INrf2MGC9454; Keap1; kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132MGC4407; KLHL19Cytosolic inhibitor of Nrf2; MGC10630; MGC1114; MGC20887
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