Human/Mouse/Rat MBP Antibody

(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat MBP
  • Source
    Monoclonal Mouse IgG1 Clone # 932908
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Purified MBP from bovine brain
    Accession # P02686
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Immunohistochemistry
    0.1-25 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat MBP by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15‑22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
MBP in Rat Cortical Stem Cells. MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces, cytoplasm, and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunohistochemistry
MBP in Rat Brain. MBP was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
MBP in Mouse Brain. MBP was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBP

Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

  • Long Name:
    Myelin Basic Protein
  • Entrez Gene IDs:
    4155 (Human); 17196 (Mouse); 24547 (Rat)
  • Alternate Names:
    Hmbpr; MBP; MGC99675; mld; Myelin A1 protein; myelin basic protein; Myelin membrane encephalitogenic protein; shi
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Human/Mouse/Rat MBP Antibody MAB42282
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Excellent
 ICC/IF Mouse Anonymous 10/23/2017
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Immunocytochemistry/Immunofluorescence Human/Mouse/Rat MBP Antibody MAB42282
Immunocytochemistry/Immunofluorescence: Human/Mouse/Rat MBP Antibody [MAB42282]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedMouse Pluripotent Stem Cells
SpeciesMouse

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