|Detection of Mouse and Rat MBP by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|MBP in Rat Cortical Stem Cells. MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces, cytoplasm, and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.|
Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.
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