Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 932908
View all MBP Primary Antibodies »

Product Specifications

Immunogen

Purified MBP from bovine brain
Accession # P02686

Specificity

Detects human, mouse, and rat MBP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat MBP Antibody

Detection of Human MBP antibody by Western Blot.

Detection of Human MBP by Western Blot.

Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse and Rat MBP antibody by Western Blot.

Detection of Mouse and Rat MBP by Western Blot.

Western blot shows lysates of mouse brain (cerebellum) tissue and rat brain (cerebellum) tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MBP at approximately 15-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MBP antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

MBP in Rat Cortical Stem Cells.

MBP was detected in immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces, cytoplasm, and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
MBP antibody in Human Brain by Immunohistochemistry (IHC-P).

MBP in Human Brain.

MBP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
MBP antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

MBP in Mouse Brain.

MBP was detected in perfusion fixed frozen sections of mouse brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
MBP antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

MBP in Rat Brain.

MBP was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat MBP Monoclonal Antibody (Catalog # MAB42282) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to myelinated fibers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Human MBP by Immunohistochemistry

Detection of Human MBP by Immunohistochemistry

Pronounced myelin loss in FTD-GRN. (A, C) Representative western blots and (B, D) densitometric quantification of PLP, CNP, MBP, beta III-tubulin ( beta III-T) and neurofilament light chain (NF-L) in (A, B) superior frontal white matter, and (C, D) superior parietal white matter from control (n = 11), FTD-C9orf72 (n = 11), and FTD-GRN (n = 6) cases. Protein levels were normalised to beta -actin or GAPDH as a loading control, and are expressed relative to the mean of the control group. (E) Representative images and (F) myelination scores from LFB staining of superior frontal gyrus white matter from control (n = 5), FTD-C9orf72 (n = 4), and FTD-GRN (n = 3) cases from which tissue fixed for < 2 weeks was available. (G) Representative ASPA (red) and MBP (green) staining in superior frontal gyrus white matter, and (H) ASPA-positive cell density. Groups were compared by one-way ANOVA with Tukey’s post-test (B,D,H) or Kruskall-Wallis test with Dunn’s post-test (F): *p < 0.05; **p < 0.01; ***p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36967384), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human MBP by Immunohistochemistry

Detection of Human MBP by Immunohistochemistry

Pronounced myelin loss in FTD-GRN. (A, C) Representative western blots and (B, D) densitometric quantification of PLP, CNP, MBP, beta III-tubulin ( beta III-T) and neurofilament light chain (NF-L) in (A, B) superior frontal white matter, and (C, D) superior parietal white matter from control (n = 11), FTD-C9orf72 (n = 11), and FTD-GRN (n = 6) cases. Protein levels were normalised to beta -actin or GAPDH as a loading control, and are expressed relative to the mean of the control group. (E) Representative images and (F) myelination scores from LFB staining of superior frontal gyrus white matter from control (n = 5), FTD-C9orf72 (n = 4), and FTD-GRN (n = 3) cases from which tissue fixed for < 2 weeks was available. (G) Representative ASPA (red) and MBP (green) staining in superior frontal gyrus white matter, and (H) ASPA-positive cell density. Groups were compared by one-way ANOVA with Tukey’s post-test (B,D,H) or Kruskall-Wallis test with Dunn’s post-test (F): *p < 0.05; **p < 0.01; ***p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36967384), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MBP by Western Blot

Detection of MBP by Western Blot

GCN2 deficiency impacts the length of myelinated segments and myelin levels. Sections (30 μm) of the brain derived from two-month-old male WT (n = 3) and genetically deficient GCN2 (GCN2−/−, n = 3) mice were obtained and processed for immunofluorescence to visualize myelin segments. (A) Representative images showing the myelin basic protein (MBP) (red) staining used as a myelin marker, the nuclei DAPI (blue), and the composed image (merge). An augmented section (white box) of the images are shown at the right (Zoom). Arrows on the zoom image show the type of MBP-positive objects quantified. (B) Images obtained in A were quantified using the Image J software (1.53t version). The plot shows the number and length of myelin segments (upper panel) and the average length (lower panel) of myelin segments found in WT and GCN2−/− mice. (C) Protein extracts were prepared from brains derived from two-month-old WT (n = 4) and GCN2−/− (n = 4) mice. MBP levels were analyzed by Western blot using alpha -Tubulin as a loading control (left). Each lane represents an individual (30 μg/lane). Densitometry analysis of the blot was performed by ImageJ to quantify the intensities of the two MBP bands and then normalized to alpha -Tubulin (right) levels. Scale bar: 100 μm. **** p < 0.00001, * p < 0.05. t-test analysis. Error bars represent the mean ± SEM. The whole Western blot membrane is shown in Supplementary Figure S1. The MBP quantification strategy is described in the “Materials and Methods” section. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40004088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat MBP Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed rat cortical stem cells differentiated for 7 days to oligodendrocytes

Immunohistochemistry

0.1-25 µg/mL
Sample: Perfusion fixed frozen sections of human brain, mouse brain, and rat brain

Western Blot

0.1 µg/mL
Sample: Human brain (cerebellum) tissue, Human brain (motor cortex) tissue, Mouse brain (cerebellum) tissue, and Rat brain (cerebellum) tissue

Reviewed Applications

Read 5 reviews rated 5 using MAB42282 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MBP

Myelin Basic Protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human); 17196 (Mouse); 24547 (Rat)

Gene Symbol

MBP

UniProt

P02686

Additional MBP Products

Product Documents for Human/Mouse/Rat MBP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat MBP Antibody

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Human/Mouse/Rat MBP Antibody

Customer Reviews for Human/Mouse/Rat MBP Antibody (5)

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  • Human/Mouse/Rat MBP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spinal cord tissue
    Species: Mouse
    Verified Customer | Posted 11/29/2021
    Human/Mouse/Rat MBP Antibody MAB42282
  • Human/Mouse/Rat MBP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: NHP brain
    Species: Mouse
    Verified Customer | Posted 08/31/2021
    Human/Mouse/Rat MBP Antibody MAB42282
  • Human/Mouse/Rat MBP Antibody
    Name: Thiruloga Chandru
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 07/27/2018
    Human/Mouse/Rat MBP Antibody MAB42282
  • Human/Mouse/Rat MBP Antibody
    Name: Jothi Jayavel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mouse iPS
    Species: Mouse
    Verified Customer | Posted 01/06/2018
    Human/Mouse/Rat MBP Antibody MAB42282
  • Human/Mouse/Rat MBP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mouse Pluripotent Stem Cells
    Species: Mouse
    Verified Customer | Posted 10/23/2017
    Human/Mouse/Rat MBP Antibody MAB42282

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