Human/Mouse/Rat p38 gamma Antibody

Catalog # Availability Size / Price Qty
MAB1347
MAB1347-SP
Detection of Human/Mouse/Rat p38 gamma  by Western Blot.
3 Images
Product Details
Citations (1)
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Human/Mouse/Rat p38 gamma Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat p38 gamma in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant p38 alpha, p38 beta, or p38δ is observed.
Source
Monoclonal Mouse IgG1 Clone # 212464
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human p38 gamma
Ala7-Leu367
Accession # P53778
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat p38? antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat p38 gamma by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38 gamma, p38d and p38a (2 ng/lane) were included. A specific band for native p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunocytochemistry p38? antibody in MCF-7 and MOLT-4 Human Cell Lines by Immunocytochemistry (ICC). View Larger

p38 gamma in MCF‑7 and MOLT‑4 Human Cell Lines. p38 gamma was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control, right panel) using Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in MCF-7 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse and Rat p38? antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse and Rat p38 gamma by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for p38 gamma at approximately 46 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 gamma

p38 mitogen-activated protein kinase (MAPK) gamma is a serine-threonine protein kinase that is also known as p38 gamma, MAPK12, stress-activated protein kinase-3 (SAPK3) and extracellular signal-regulated kinase-6 (ERK6).

Entrez Gene IDs
6300 (Human)
Alternate Names
EC 2.7.11; ERK3; ERK-6; ERK6MAPK 12; Extracellular signal-regulated kinase 6; MAP kinase 12; MAP kinase p38 gamma; MAPK12; mitogen-activated protein kinase 12; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 gamma; p38 gamma; p38gamma; PRKM12; SAPK-3; SAPK3EC 2.7.11.24; Stress-activated protein kinase 3

Product Datasheets

Citation for Human/Mouse/Rat p38 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Differential tissue expression and activation of p38 MAPK alpha, beta, gamma, and delta isoforms in rheumatoid arthritis.
    Authors: Korb A, Tohidast-Akrad M, Cetin E, Axmann R, Smolen J, Schett G
    Arthritis Rheum., 2006;54(9):2745-56.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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