Detection of Human/Mouse/Rat p38 gamma by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line myoblast cell line and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human p38 beta, p38 gamma, p38δ and p38 alpha (2 ng/lane) were included. A specific band for native p38 gamma was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Mouse and Rat p38 gamma by Simple WesternTM.
Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and PC‑12 rat adrenal pheochromocytoma cell line, loaded at 0.5 mg/mL. A specific band was detected for p38 gamma at approximately 46 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat p38 gamma Monoclonal Antibody (Catalog # MAB1347). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p38 gamma
p38 mitogen-activated protein kinase (MAPK) gamma is a serine-threonine protein kinase that is also known as p38 gamma, MAPK12, stress-activated protein kinase-3 (SAPK3) and extracellular signal-regulated kinase-6 (ERK6).
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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