Human/Mouse/Rat p38 gamma Antibody

Detection of Mouse p38 gamma by Western Blot

Muscle-specific deletion of the p38 alpha, p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha, p38 beta, and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse p38 gamma by Western Blot

Muscle-specific deletion of the p38 alpha, p38 beta or p38 gamma gene does not affect endurance exercise-induced fiber-type transformation. Wild type, p38 alpha, p38 beta, and p38 gamma MKO mice were subjected to 4 weeks of voluntary running (Ex) or sedentary cage activity (Sed) followed by fiber-type and immunoblot analyses in plantaris muscles. A) PCR of genomic DNA with the appropriate primers (see Materials and Methods) for the loxP flanked p38 alleles in wild type (WT), heterozygous (+/−) and homozygous (−/−) mice with loxP-flanked p38 alleles. Only the homozygous mice with the Cre transgene (not shown) were considered p38 muscle-specific knockout (MKO) mice; B) Immunoblot analysis for p38 alpha and p38 gamma protein in plantaris muscles of p38 MKO mice in comparison with the wild type littermates. alpha -tubulin was used as a loading control; and C) Images of immunofluorescence staining of plantaris muscle sections with antibodies against myosin heavy chain IIb (Green), IIa (Blue) and I (Red). Appreciable increases in the percentage of type IIa fibers with concurrent decreases in type IIb fibers are noted in Ex group compared with Sed group among all four genetic backgrounds. The quantitative data is presented in Table 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/19936205), licensed under a CC-BY license. Not internally tested by R&D Systems.