Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PKC alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC alpha
Lys604-Val672
Accession # P17252

Specificity

Detects endogenous human, mouse, and rat PKC alpha in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKC alpha Antibody

Detection of Human/Mouse/Rat PKCa antibody by Western Blot.

Detection of Human/Mouse/Rat PKC alpha by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos-2 human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKCa at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PKCa antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

PKC alpha in Human Breast Cancer Tissue.

PKCa was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human/Mouse/Rat PKCa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human PKC alpha by Simple WesternTM.

Simple Western shows lysates of Saos‑2 human osteosarcoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC alpha at approximately 84 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat PKC alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Human/Mouse/Rat PKC alpha Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Simple Western

10 µg/mL
Sample: Saos-2 human osteosarcoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Saos-2 human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cells

Reviewed Applications

Read 3 reviews rated 5 using AF5340 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKC alpha

PKC alpha (Protein kinase C alpha) is an 80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is widely expressed, and serves multiple cell-specific functions. PKC alpha is activated by increased diacylglycerol and intracellular calcium, and translocates to several sites such as the Golgi, nucleus and plasma membrane. Human PKC alpha is 672 amino acids (aa) in length. It contains two zinc finger regions (aa 36‑151), a protein kinase domain (aa 339‑557), and an AGC kinase region (aa 598‑668). Phosphorylations on Thr497, Thr638 and Ser657 are necessary for kinase activity. Over aa 604‑672, human PKC alpha shows 100% aa identity to mouse PKC alpha.

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Entrez Gene IDs

5578 (Human); 18750 (Mouse); 24680 (Rat); 447031 (Xenopus)

Gene Symbol

PRKCA

UniProt

P17252

Additional PKC alpha Products

Product Documents for Human/Mouse/Rat PKC alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat PKC alpha Antibody

For research use only

Related Research Areas

Diabetes
Intracellular Kinases
PDGF Family

Citations for Human/Mouse/Rat PKC alpha Antibody

Customer Reviews for Human/Mouse/Rat PKC alpha Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat PKC alpha Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse Retinal Cells
    Species: Mouse
    Verified Customer | Posted 01/21/2021
    Isolated retinal cells stained with gt-PKCalpha (AF5340, 1:1000; cyan); Labelling conforms to rod bipolar cell morphology, comparable to PKCalpha antibodies from mouse and rabbit.
    Papain dissociation of retina tissue, PFA fixation and staining for isolated rod bipolar cells with gt-PKCalpha (AF5340, 1:1000) + dk-a-gt DyLight594 (1:250); PKCalpha labelling conforms to rod bipolar cell morphology.
    Human/Mouse/Rat PKC alpha Antibody AF5340
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Human/Mouse/Rat PKC alpha Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: C57BL/6 mouse retina
    Species: Mouse
    Verified Customer | Posted 01/21/2021
    Mouse retina cryosection stained with gt-PKCalpha (AF5340, 1:250; cyan) and counterstained with DAPI (grey). PKCalpha labelling conforms to the rod bipolar cell morphology, comparable to established PKCalpha marker antibodies.
    gt-PKCalpha (AF5340, 1:250) + dk-a-gt DyLight594 (1:250) PFA-fixed tissue
    Human/Mouse/Rat PKC alpha Antibody AF5340
  • Human/Mouse/Rat PKC alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Endothelial
    Species: Mouse
    Verified Customer | Posted 10/11/2019
    Human/Mouse/Rat PKC alpha Antibody AF5340

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Protocols

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