Detection of Human/Mouse/Rat PKC alpha by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Saos‑2 human osteosarcoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC alpha at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKC alpha in Human Breast Cancer Tissue.
PKC alpha was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human/Mouse/Rat PKC alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5340) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC alpha
PKC alpha (Protein kinase C alpha) is an 80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is widely expressed, and serves multiple cell-specific functions. PKC alpha is activated by increased diacylglycerol and intracellular calcium, and translocates to several sites such as the Golgi, nucleus and plasma membrane. Human PKC alpha is 672 amino acids (aa) in length. It contains two zinc finger regions (aa 36‑151), a protein kinase domain (aa 339‑557), and an AGC kinase region (aa 598‑668). Phosphorylations on Thr497, Thr638 and Ser657 are necessary for kinase activity. Over aa 604‑672, human PKC alpha shows 100% aa identity to mouse PKC alpha.
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