Human/Mouse/Rat RAGE/AGER Antibody
Human/Mouse/Rat RAGE/AGER Antibody Summary
Gln24-Ala344
Accession # Q15109
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human, Mouse, and Rat RAGE by Western Blot. Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
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RAGE in Human Alzheimer's Disease Brain. RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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RAGE in Human Lung. RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RAGE/AGER
Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules. AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins.
The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain. The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known. RAGE is highly expressed in the embryonic central nervous system. In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes. The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression. RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders.
Product Datasheets
Citations for Human/Mouse/Rat RAGE/AGER Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
22
Citations: Showing 1 - 10
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Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
Authors: P Kadur Laks, V Sontake, A Tata, Y Kobayashi, L Macadlo, K Okuda, AS Conchola, S Nakano, S Gregory, LA Miller, JR Spence, JF Engelhardt, RC Boucher, JR Rock, SH Randell, PR Tata
Nature, 2022;604(7904):111-119.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
A Dunaliella salina Extract Counteracts Skin Aging under Intense Solar Irradiation Thanks to Its Antiglycation and Anti-Inflammatory Properties
Authors: F Havas, S Krispin, M Cohen, E Loing, M Farge, T Suere, J Attia-Vign
Marine Drugs, 2022;20(2):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Inhibition of the Receptor for Advanced Glycation End Products Enhances the Cytotoxic Effect of Gemcitabine in Murine Pancreatic Tumors
Authors: P Swami, KA O'Connell, S Thiyagaraj, A Crawford, P Patil, P Radhakrish, S Shin, TC Caffrey, J Grunkemeye, T Neville, SW Vetter, MA Hollingswo, E Leclerc
Biomolecules, 2021;11(4):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Soluble Receptor for Advanced Glycation End-products regulates age-associated Cardiac Fibrosis
Authors: F Scavello, F Zeni, G Milano, F Macrì, S Castiglion, E Zuccolo, A Scopece, G Pezone, CC Tedesco, P Nigro, G Degani, E Gambini, F Veglia, L Popolo, G Pompilio, GI Colombo, ME Bianchi, A Raucci
International journal of biological sciences, 2021;17(10):2399-2416.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2
Authors: J Youk, T Kim, KV Evans, YI Jeong, Y Hur, SP Hong, JH Kim, K Yi, SY Kim, KJ Na, T Bleazard, HM Kim, M Fellows, KT Mahbubani, K Saeb-Parsy, SY Kim, YT Kim, GY Koh, BS Choi, YS Ju, JH Lee
Cell Stem Cell, 2020;27(6):905-919.e10.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
Cell Stem Cell, 2020;0(0):.
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Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging
Authors: F Scavello, F Zeni, CC Tedesco, E Mensà, F Veglia, AD Procopio, AR Bonfigli, F Olivieri, A Raucci
Aging (Albany NY), 2019;11(6):1648-1663.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
EMC3 coordinates surfactant protein and lipid homeostasis required for respiration
Authors: X Tang, JM Snowball, Y Xu, CL Na, TE Weaver, G Clair, JE Kyle, EM Zink, C Ansong, W Wei, M Huang, X Lin, JA Whitsett
J. Clin. Invest., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis
PLoS ONE, 2016;11(9):e0163330.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE
PLoS ONE, 2016;11(9):e0153832.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
Cell Death Differ, 2015;22(7):1219-30.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
Authors: Iotzova-Weiss G, Dziunycz P, Freiberger S, Lauchli S, Hafner J, Vogl T, French L, Hofbauer G
PLoS ONE, 2015;10(3):e0120971.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
Authors: Sessa L, Gatti E, Zeni F, Antonelli A, Catucci A, Koch M, Pompilio G, Fritz G, Raucci A, Bianchi M
PLoS ONE, 2014;9(1):e86903.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFbeta1.
Authors: Basso D, Bozzato D, Padoan A, Moz S, Zambon C, Fogar P, Greco E, Scorzeto M, Simonato F, Navaglia F, Fassan M, Pelloso M, Dupont S, Pedrazzoli S, Fassina A, Plebani M
Cell Commun Signal, 2014;12(0):20.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
Authors: Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H
PLoS ONE, 2014;9(4):e94118.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
Authors: Winden D, Barton D, Betteridge B, Bodine J, Jones C, Rogers G, Chavarria M, Wright A, Jergensen Z, Jimenez F, Reynolds P
Respir Res, 2014;15(0):129.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
Authors: Monden, Masayo, Koyama, Hidenori, Otsuka, Yoshiko, Morioka, Tomoaki, Mori, Katsuhit, Shoji, Takuhito, Mima, Yohei, Motoyama, Koka, Fukumoto, Shinya, Shioi, Atsushi, Emoto, Masanori, Yamamoto, Yasuhiko, Yamamoto, Hiroshi, Nishizawa, Yoshiki, Kurajoh, Masafumi, Yamamoto, Tetsuya, Inaba, Masaaki
Diabetes, 2013;62(2):478-89.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
Authors: Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
Fertil. Steril., 2010;94(5):1639.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
Authors: Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
Am. J. Physiol., Cell Physiol., 2010;299(1):C97-110.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.
Authors: Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, Steger K, Stitt A, McClure N
Hum. Reprod., 2007;22(8):2169-77.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
Authors: Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ
Am. J. Respir. Cell Mol. Biol., 2007;36(6):661-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
Authors: Zen K, Chen CX, Chen YT, Wilton R, Liu Y
J. Immunol., 2007;178(4):2483-90.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
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