Human/Mouse/Rat RAGE/AGER Antibody

Catalog # Availability Size / Price Qty
AF1145
AF1145-SP
Detection of Human, Mouse, and Rat RAGE by Western Blot.
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Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse/Rat RAGE/AGER Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human RAGE in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RAGE
Gln24-Ala344
Accession # Q15109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
0.5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 8-40 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human RAGE Fc Chimera (Catalog # 1145-RG) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat RAGE by Western Blot. View Larger

Detection of Human, Mouse, and Rat RAGE by Western Blot. Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human RAGE by Western Blot. View Larger

Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.

Immunohistochemistry RAGE in Human Alzheimer's Disease Brain. View Larger

RAGE in Human Alzheimer's Disease Brain. RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry RAGE in Human Lung. View Larger

RAGE in Human Lung. RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules. AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins.

The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain. The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known. RAGE is highly expressed in the embryonic central nervous system. In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes. The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression. RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders.

Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

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Citations for Human/Mouse/Rat RAGE/AGER Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Human distal lung maps and lineage hierarchies reveal a bipotent progenitor
    Authors: P Kadur Laks, V Sontake, A Tata, Y Kobayashi, L Macadlo, K Okuda, AS Conchola, S Nakano, S Gregory, LA Miller, JR Spence, JF Engelhardt, RC Boucher, JR Rock, SH Randell, PR Tata
    Nature, 2022;604(7904):111-119.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. A Dunaliella salina Extract Counteracts Skin Aging under Intense Solar Irradiation Thanks to Its Antiglycation and Anti-Inflammatory Properties
    Authors: F Havas, S Krispin, M Cohen, E Loing, M Farge, T Suere, J Attia-Vign
    Marine Drugs, 2022;20(2):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Inhibition of the Receptor for Advanced Glycation End Products Enhances the Cytotoxic Effect of Gemcitabine in Murine Pancreatic Tumors
    Authors: P Swami, KA O'Connell, S Thiyagaraj, A Crawford, P Patil, P Radhakrish, S Shin, TC Caffrey, J Grunkemeye, T Neville, SW Vetter, MA Hollingswo, E Leclerc
    Biomolecules, 2021;11(4):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Soluble Receptor for Advanced Glycation End-products regulates age-associated Cardiac Fibrosis
    Authors: F Scavello, F Zeni, G Milano, F Macrì, S Castiglion, E Zuccolo, A Scopece, G Pezone, CC Tedesco, P Nigro, G Degani, E Gambini, F Veglia, L Popolo, G Pompilio, GI Colombo, ME Bianchi, A Raucci
    International journal of biological sciences, 2021;17(10):2399-2416.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2
    Authors: J Youk, T Kim, KV Evans, YI Jeong, Y Hur, SP Hong, JH Kim, K Yi, SY Kim, KJ Na, T Bleazard, HM Kim, M Fellows, KT Mahbubani, K Saeb-Parsy, SY Kim, YT Kim, GY Koh, BS Choi, YS Ju, JH Lee
    Cell Stem Cell, 2020;27(6):905-919.e10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
    Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
    Cell Stem Cell, 2020;0(0):.
  7. Modulation of soluble receptor for advanced glycation end-products (RAGE) isoforms and their ligands in healthy aging
    Authors: F Scavello, F Zeni, CC Tedesco, E Mensà, F Veglia, AD Procopio, AR Bonfigli, F Olivieri, A Raucci
    Aging (Albany NY), 2019;11(6):1648-1663.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration
    Authors: X Tang, JM Snowball, Y Xu, CL Na, TE Weaver, G Clair, JE Kyle, EM Zink, C Ansong, W Wei, M Huang, X Lin, JA Whitsett
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis
    PLoS ONE, 2016;11(9):e0163330.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  10. The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE
    PLoS ONE, 2016;11(9):e0153832.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015;22(7):1219-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
    Authors: Iotzova-Weiss G, Dziunycz P, Freiberger S, Lauchli S, Hafner J, Vogl T, French L, Hofbauer G
    PLoS ONE, 2015;10(3):e0120971.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
    Authors: Sessa L, Gatti E, Zeni F, Antonelli A, Catucci A, Koch M, Pompilio G, Fritz G, Raucci A, Bianchi M
    PLoS ONE, 2014;9(1):e86903.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFbeta1.
    Authors: Basso D, Bozzato D, Padoan A, Moz S, Zambon C, Fogar P, Greco E, Scorzeto M, Simonato F, Navaglia F, Fassan M, Pelloso M, Dupont S, Pedrazzoli S, Fassina A, Plebani M
    Cell Commun Signal, 2014;12(0):20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
    Authors: Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H
    PLoS ONE, 2014;9(4):e94118.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
    Authors: Winden D, Barton D, Betteridge B, Bodine J, Jones C, Rogers G, Chavarria M, Wright A, Jergensen Z, Jimenez F, Reynolds P
    Respir Res, 2014;15(0):129.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
    Authors: Monden, Masayo, Koyama, Hidenori, Otsuka, Yoshiko, Morioka, Tomoaki, Mori, Katsuhit, Shoji, Takuhito, Mima, Yohei, Motoyama, Koka, Fukumoto, Shinya, Shioi, Atsushi, Emoto, Masanori, Yamamoto, Yasuhiko, Yamamoto, Hiroshi, Nishizawa, Yoshiki, Kurajoh, Masafumi, Yamamoto, Tetsuya, Inaba, Masaaki
    Diabetes, 2013;62(2):478-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
    Authors: Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
    Fertil. Steril., 2010;94(5):1639.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
    Authors: Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
    Am. J. Physiol., Cell Physiol., 2010;299(1):C97-110.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  20. Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.
    Authors: Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, Steger K, Stitt A, McClure N
    Hum. Reprod., 2007;22(8):2169-77.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  21. Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
    Authors: Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ
    Am. J. Respir. Cell Mol. Biol., 2007;36(6):661-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
    Authors: Zen K, Chen CX, Chen YT, Wilton R, Liu Y
    J. Immunol., 2007;178(4):2483-90.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Human/Mouse/Rat RAGE Antibody
By Balaji Mahender on 12/06/2017
Application: ELISA Sample Tested: EDTA Plasma,Heparin Plasma,Platelet-poor EDTA Plasma Species: Human