Human/Mouse/Rat RAGE Antibody AF1145: R&D Systems

Human/Mouse/Rat RAGE Antibody

(14 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human RAGE in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity recombinant canine RAGE is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human RAGE
    Gln24-Ala344
    Accession # Q15109
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    0.5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 4-12 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human RAGE Fc Chimera (Catalog # 1145-RG) coated at 5 µg/mL (100 µL/well). At 80 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat RAGE by Western Blot. Western blot shows lysates of human, mouse, and rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately
45-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue and HEK001 human epidermal keratinocyte cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted using Immunoblot Buffer Group 5.
Immunohistochemistry
RAGE in Human Alzheimer's Disease Brain. RAGE was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cerebellum) using 15 µg/mL Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
RAGE in Human Lung. RAGE was detected in immersion fixed paraffin-embedded sections of human lung using Goat Anti-Human/Mouse/Rat RAGE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1145) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RAGE

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules. AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins.

The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain. The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known. RAGE is highly expressed in the embryonic central nervous system. In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes, and hepatocytes. The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression. RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion, and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders.

  • Long Name:
    Receptor for Advanced Glycation End Products
  • Entrez Gene IDs:
    177 (Human); 11596 (Mouse); 81722 (Rat)
  • Alternate Names:
    AGER; EC 2.7.11.22; MOK protein kinase; MOKMAPK/MAK/MRK overlapping kinase; RAGE; RAGE-1; RAGE1renal cell carcinoma antigen (MOK protein kinase); Renal tumor antigen 1; renal tumor antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. The Mouse-Specific Splice Variant mRAGE_v4 Encodes a Membrane-Bound RAGE That Is Resistant to Shedding and Does Not Contribute to the Production of Soluble RAGE
    PLoS ONE, 2016;11(9):e0153832.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. The Role of Neutrophil Proteins on the Amyloid Beta-RAGE Axis
    PLoS ONE, 2016;11(9):e0163330.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  3. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015;22(7):1219-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. S100A8/A9 stimulates keratinocyte proliferation in the development of squamous cell carcinoma of the skin via the receptor for advanced glycation-end products.
    Authors: Iotzova-Weiss G, Dziunycz P, Freiberger S, Lauchli S, Hafner J, Vogl T, French L, Hofbauer G
    PLoS ONE, 2015;10(3):e0120971.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. The receptor for advanced glycation end-products (RAGE) is only present in mammals, and belongs to a family of cell adhesion molecules (CAMs).
    Authors: Sessa L, Gatti E, Zeni F, Antonelli A, Catucci A, Koch M, Pompilio G, Fritz G, Raucci A, Bianchi M
    PLoS ONE, 2014;9(1):e86903.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Inflammation and pancreatic cancer: molecular and functional interactions between S100A8, S100A9, NT-S100A8 and TGFbeta1.
    Authors: Basso D, Bozzato D, Padoan A, Moz S, Zambon C, Fogar P, Greco E, Scorzeto M, Simonato F, Navaglia F, Fassan M, Pelloso M, Dupont S, Pedrazzoli S, Fassina A, Plebani M
    Cell Commun Signal, 2014;12(0):20.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation.
    Authors: Winden D, Barton D, Betteridge B, Bodine J, Jones C, Rogers G, Chavarria M, Wright A, Jergensen Z, Jimenez F, Reynolds P
    Respir Res, 2014;15(0):129.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  8. Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
    Authors: Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H
    PLoS ONE, 2014;9(4):e94118.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Receptor for advanced glycation end products regulates adipocyte hypertrophy and insulin sensitivity in mice: involvement of Toll-like receptor 2.
    Authors: Monden, Masayo, Koyama, Hidenori, Otsuka, Yoshiko, Morioka, Tomoaki, Mori, Katsuhit, Shoji, Takuhito, Mima, Yohei, Motoyama, Koka, Fukumoto, Shinya, Shioi, Atsushi, Emoto, Masanori, Yamamoto, Yasuhiko, Yamamoto, Hiroshi, Nishizawa, Yoshiki, Kurajoh, Masafumi, Yamamoto, Tetsuya, Inaba, Masaaki
    Diabetes, 2013;62(2):478-89.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  10. Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
    Authors: Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
    Am. J. Physiol., Cell Physiol., 2010;299(1):C97-110.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA Development
  11. In vitro effects of atorvastatin on lipopolysaccharide-induced gene expression in endometriotic stromal cells.
    Authors: Sharma I, Dhawan V, Mahajan N, Saha SC, Dhaliwal LK
    Fertil. Steril., 2010;94(5):1639.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.
    Authors: Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, Steger K, Stitt A, McClure N
    Hum. Reprod., 2007;22(8):2169-77.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  13. Differentiated human alveolar epithelial cells and reversibility of their phenotype in vitro.
    Authors: Wang J, Edeen K, Manzer R, Chang Y, Wang S, Chen X, Funk CJ, Cosgrove GP, Fang X, Mason RJ
    Am. J. Respir. Cell Mol. Biol., 2007;36(6):661-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
    Authors: Zen K, Chen CX, Chen YT, Wilton R, Liu Y
    J. Immunol., 2007;178(4):2483-90.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 14 Citations
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Summary

ApplicationELISA
Sample TestedEDTA Plasma,Platelet-poor EDTA Plasma,Heparin Plasma
SpeciesHuman

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