|Detection of Human/Mouse/Rat Rheb by Western Blot. Western blot shows lysates of NRK rat normal kidney cell line, Ramos human Burkitt's lymphoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Rheb Monoclonal Antibody (Catalog # MAB3426) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rheb at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Ras homolog enriched in brain (Rheb) is a novel member of the Ras superfamily of GTP-binding proteins involved in cell growth and cell cycle progression. Expressed in a wide range of tissues, Rheb was initially identified from its induction in rat hippocampal granule cells by seizures and NMDA-dependent synaptic activity. A complex containing the tuberous sclerosis gene products TSC1 and TSC2 functions as a GTPase-activating protein (GAP) for Rheb.
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