The STAT family of transcription factors, first described as key mediators of interferon signal transduction, are now recognized as signaling factors downstream of many cytokines, growth factors, and hormones. At least seven STATs exist: STAT1, 2, 3, 4, 5a, 5b, and 6. They are responsible for an array of cellular activities including regulating growth, survival, differentiation, motility, and the immune response. They are characterized structurally by the presence of the Src homology 2 (SH2) domain, and a C-terminal tyrosine phosphorylation site. Upon ligand binding, they are recruited to the activated receptor, via their SH2 domains, to receptor-associated tyrosine kinases of the Janus kinase family (JAK). Once phosphorylated by JAK, STATS are free to hetero- or homo-dimerize, and translocate to the nucleus where they bind DNA to regulate gene transcription.
Key Product Details
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Label
Antibody Source
Product Specifications
Immunogen
Arg688-Met770
Accession # U06922
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human/Mouse/Rat STAT3 Antibody
Detection of STAT3-regulated Genes by Chromatin Immunoprecipitation.
Jurkat human acute T cell leukemia cell line treated with 1000 U/mL Recombinant Human IFN-a Universal Type I (Catalog # 11200-1) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT3/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR.
Immunoprecipitation of Human STAT3.
STAT3 was immunoprecipitated from 500 µg of Daudi human Burkitt's lymphoma cell line cytoplasmic and nuclear lystates following incubation with 2 µg Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) for overnight at 4 ºC. STAT3-antibody complexes were absorbed using Protein G agarose (Invitrogen). Immunoprecipitated STAT3 was detected by Western blot using 0.1 µg/mL Human STAT3 Monoclonal Antibody (Catalog # MAB1799). View our recommended buffer recipes for immunoprecipitation.
STAT3 in D3 Mouse Cell Line.
STAT3 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/Mouse/Rat STAT3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1799) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Tubulin was also detected using Tubulin Antibody (Catalog # NB600-506; Novus Biologicals) and stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015). Cells were counterstained with DAPI (blue). Specific staining of STAT3 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Applications for Human/Mouse/Rat STAT3 Antibody
Chromatin Immunoprecipitation (ChIP)
Sample: Human IFN‑ alpha treated Jurkat human acute T cell leukemia cell line chromatin, c-myc promoter detected by standard PCR.
Immunocytochemistry
Sample: Immersion fixed mouse D3 embryonic stem cells
Immunoprecipitation
Sample: Daudi human Burkitt's lymphoma cell line, see our available Western blot detection antibodies
Reviewed Applications
Read 1 review rated 5 using AF1799 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: STAT3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional STAT3 Products
Product Documents for Human/Mouse/Rat STAT3 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat STAT3 Antibody
For research use only
Citations for Human/Mouse/Rat STAT3 Antibody
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Application: ImmunoprecipitationSample Tested: IPS2 induced pluripotent stem cellsSpecies: HumanVerified Customer | Posted 01/17/2018
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ChIP Protocol Video
- Chromatin Immunoprecipitation (ChIP) Protocol
- Chromatin Immunoprecipitation Protocol
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Associated Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-2 Signaling Pathways
IL-7 Signaling Pathways
IL-9 Signaling Pathways
IL-21 Signaling Pathways
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Jak/STAT Signaling Pathway
Th1 Differentiation Pathway
Th17 Differentiation Pathway
VEGF - VEGF R2 Signaling Pathways
