Detection of Human, Mouse, and Rat STIM1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat STIM1 Monoclonal Antibody (Catalog # MAB6976) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STIM1 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane
protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of
calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the
plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel
Orai1, increasing Ca2+ influx. The human STIM-1
extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding
motif and a SAM (sterile a-motif)
multimerization domain. A potential isoform is truncated at aa 491. Defects in
STIM1 are the cause of immune dysfunction with T-cell inactivation due to
calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen,
human STIM1 shares 95% amino acid identity with mouse and rat STIM1.
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