Vinculin in HeLa Human Cell Line.
Vinculin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Vinculin in Human Uterus.
Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB68961) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.
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