SALL4 Antibody (651839)

R&D Systems | Catalog # MAB6374

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 651839
View all SALL4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SALL4
Lys96-Gly359
Accession # Q9UJQ4

Specificity

Detects endogenous human and mouse SALL4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SALL4 Antibody (651839)

Detection of Human SALL4 antibody by Western Blot.

Detection of Human SALL4 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for SALL4 Antibody (651839)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Western Blot

0.025 µg/mL
Sample: BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB6374 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SALL4

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C2H2-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

Long Name

Sal-like 4

Alternate Names

DRRS, HSAL4, ZNF797

Entrez Gene IDs

57167 (Human); 99377 (Mouse)

Gene Symbol

SALL4

UniProt

Q9UJQ4 (Human)

Additional SALL4 Products

Product Documents for SALL4 Antibody (651839)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SALL4 Antibody (651839)

For research use only

Citations for SALL4 Antibody (651839)

Customer Reviews for SALL4 Antibody (651839) (2)

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2 Customer Ratings
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  • Human/Mouse SALL4 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Whole Cells
    Species: Human
    Verified Customer | Posted 01/20/2023
    Jurkat whole cell lysate, 0.6 mg/ml, Anti Sall4 1:1000 dilution, 12-230 kDa Protein Simple detection module
    SALL4 Antibody (651839) MAB6374
  • Human/Mouse SALL4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Testis tissue
    Species: Mouse
    Verified Customer | Posted 07/07/2022
    SALL4 Antibody (651839) MAB6374

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