Human/Mouse SALL4 Antibody

Catalog # Availability Size / Price Qty
MAB6374
MAB6374-SP
Detection of Human SALL4 by Western Blot.
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Human/Mouse SALL4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse SALL4 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 651839
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SALL4
Lys96-Gly359
Accession # Q9UJQ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
0.025 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human testis

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SALL4 antibody by Western Blot. View Larger

Detection of Human SALL4 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.025 µg/mL of Mouse Anti-Human/Mouse SALL4 Monoclonal Antibody (Catalog # MAB6374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SALL4 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SALL4

SALL4 (SAL-like protein 4) is a 1324 amino acid (aa) member of the SAL protein family that possess multiple C2H2-type zinc-fingers. It is a transcriptional repressor when associated with histone deacetylase and a transcriptional activator of the Wnt pathway in its native form. It contributes to generation of induced pluripotent stem cells and to axis formation during development. It is frequently overexpressed in germ cell tumors and acute myeloid leukemias. Mutations of human SALL4 can result in conditions such as Okihiro, Duane Radial Ray, or Holt-Oram syndromes. The region used as an immunogen is common to both SALL4 and the ubiquitous 617 aa isoform, SALL4B; it shares 76% aa identity between human and mouse.

Long Name
Sal-like 4
Entrez Gene IDs
57167 (Human); 99377 (Mouse)
Alternate Names
dJ1112F19.1; DRRS; HSAL4; MGC133050; SALL4; sal-like 4 (Drosophila); sal-like protein 4; Zinc finger protein 797; Zinc finger protein SALL4; ZNF797; ZNF797sal (Drosophila)-like 4

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse SALL4 Antibody

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Human/Mouse SALL4 Antibody
By Anonymous on 07/07/2022
Application: IHC Sample Tested: Testis tissue Species: Mouse