Human/Mouse Wnt-11 Antibody

Catalog # Availability Size / Price Qty
AF2647
AF2647-SP
Human/Mouse Wnt-11 Antibody in Data
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Product Details
Citations (1)
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Human/Mouse Wnt-11 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse Wnt-11 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt‑11 peptide
Leu39-Ala79 and Lys225-Arg297
Accession # Q059Y4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Wnt‑11
Immunohistochemistry
5-15 µg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Wnt-11 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC). View Larger

Wnt‑11 in LNCaP Human Cell Line. Wnt-11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cell signaling molecules that play important roles in vertebrate development. Mouse Wnt-11 shares 97% amino acid sequence identity with its human and rat homolog.

Long Name
Wingless-type MMTV Integration Site Family, Member 11
Entrez Gene IDs
7481 (Human); 22411 (Mouse)
Alternate Names
HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11; Wnt-11

Product Datasheets

Citation for Human/Mouse Wnt-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension.
    Authors: Laumanns IP, Fink L, Wilhelm J, Wolff JC, Mitnacht-Kraus R, Graef-Hoechst S, Stein MM, Bohle RM, Klepetko W, Hoda MA, Schermuly RT, Grimminger F, Seeger W, Voswinckel R
    Am. J. Respir. Cell Mol. Biol., 2009;40(6):683-91.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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