Detects mouse Wnt-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) Wnt-3A is observed and less than 1% cross-reactivity with rmWnt-5A is observed.
Polyclonal Goat IgG
E. coli-derived recombinant mouse Wnt‑11 peptide Leu39-Ala79 and Lys225-Arg297 Accession # Q059Y4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Mouse Wnt‑11
Immersion fixed frozen sections of mouse embryo (E13-15)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Wnt‑11 in LNCaP Human Cell Line. Wnt‑11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt‑11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Wnt-11 belongs to the Wnt family of secreted, highly conserved, cell
signaling molecules that play important roles in vertebrate
development. Mouse Wnt-11 shares 97% amino acid sequence identity with
its human and rat homolog.
Wingless-type MMTV Integration Site Family, Member 11
Entrez Gene IDs:
7481 (Human); 22411 (Mouse)
HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.