Human/Mouse Wnt-11 Antibody

Catalog #: AF2647
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2647
AF2647-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Wnt-11 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Wnt-11 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Wnt‑11 peptide
Leu39-Ala79 and Lys225-Arg297
Accession # Q059Y4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Wnt‑11

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13-15)

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Wnt‑11 in LNCaP Human Cell Line. Wnt-11 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Mouse Wnt-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2647) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-11

Wnt-11 belongs to the Wnt family of secreted, highly conserved, cell signaling molecules that play important roles in vertebrate development. Mouse Wnt-11 shares 97% amino acid sequence identity with its human and rat homolog.

Long Name

Wingless-type MMTV Integration Site Family, Member 11

Entrez Gene IDs

7481 (Human); 22411 (Mouse)

Alternate Names

HWNT11; MGC141946; MGC141948; protein Wnt-11; wingless-type MMTV integration site family, member 11; Wnt11; Wnt-11

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse Wnt-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

The noncanonical WNT pathway is operative in idiopathic pulmonary arterial hypertension.
Authors: Laumanns IP, Fink L, Wilhelm J, Wolff JC, Mitnacht-Kraus R, Graef-Hoechst S, Stein MM, Bohle RM, Klepetko W, Hoda MA, Schermuly RT, Grimminger F, Seeger W, Voswinckel R
Am. J. Respir. Cell Mol. Biol., 2009;40(6):683-91.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P