Human/Mouse XIAP Antibody

Name: Human/Mouse XIAP Antibody
Brand: R&D Systems
Rating: 4 (1 reviews)

MAB822 5 Citations 1 Review Datasheet / COA / SDS

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MAB822
MAB822-SP

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Detection of Human/Mouse XIAP by Western Blot.

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Product Details

Citations (5)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse XIAP Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse XIAP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) cIAP-1 or rhcIAP-2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 117320

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human XIAP
Met1-Ser497
Accession # P98170

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Knockout Validated

XIAP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in XIAP knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human/Mouse XIAP by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human XIAP Full Length (Catalog # 822-XF) and recombinant mouse XIAP (5 ng/lane) were included. A specific band for XIAP was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.

Knockout Validated

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Western Blot Shows Human XIAP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for XIAP at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: XIAP

XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.

Long Name

X-linked Inhibitor of Apoptosis

Entrez Gene IDs

331 (Human); 11798 (Mouse); 63879 (Rat)

Alternate Names

API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse XIAP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes
Authors: S Gradzka, OS Thomas, O Kretz, A Haimovici, L Vasilikos, WW Wong, G Häcker, IE Gentle
Cell Death Dis, 2018;9(5):529.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB
Evaluating the mechanism and therapeutic potential of PTC-028, a novel inhibitor of BMI-1 function in ovarian cancer
Authors: A Dey, X Xiong, A Crim, SKD Dwivedi, S Banerjee M, P Mukherjee, L Cao, N Sydorenko, R Baiazitov, YC Moon, M Dumble, T Davis, R Bhattachar
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer.
Authors: Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N
Int J Oncol, 2014;45(3):1043-50.
Species: Human
Sample Types: Protein
Applications: WB
MUC16 mucin (CA125) attenuates TRAIL-induced apoptosis by decreasing TRAIL receptor R2 expression and increasing c-FLIP expression.
Authors: Matte I, Lane D, Boivin M, Rancourt C, Piche A
BMC Cancer, 2014;14(0):234.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals.
Authors: Schoenmann Z, Assa-Kunik E, Tiomny S, Minis A, Haklai-Topper L, Arama E, Yaron A
J. Neurosci., 2010;30(18):6375-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB