Human/Mouse XIAP Antibody
Human/Mouse XIAP Antibody Summary
Met1-Ser497
Accession # P98170
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Human/Mouse XIAP by Western Blot. Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibrosarcoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human XIAP Full Length (Catalog # 822-XF) and recombinant mouse XIAP (5 ng/lane) were included. A specific band for XIAP was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
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Western Blot Shows Human XIAP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and XIAP knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse XIAP Monoclonal Antibody (Catalog # MAB822) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for XIAP at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: XIAP
XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits Caspase-3 and Caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.
Product Datasheets
Citations for Human/Mouse XIAP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes
Authors: S Gradzka, OS Thomas, O Kretz, A Haimovici, L Vasilikos, WW Wong, G Häcker, IE Gentle
Cell Death Dis, 2018;9(5):529.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Evaluating the mechanism and therapeutic potential of PTC-028, a novel inhibitor of BMI-1 function in ovarian cancer
Authors: A Dey, X Xiong, A Crim, SKD Dwivedi, S Banerjee M, P Mukherjee, L Cao, N Sydorenko, R Baiazitov, YC Moon, M Dumble, T Davis, R Bhattachar
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer.
Authors: Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N
Int J Oncol, 2014;45(3):1043-50.
Species: Human
Sample Types: Protein
Applications: Western Blot -
MUC16 mucin (CA125) attenuates TRAIL-induced apoptosis by decreasing TRAIL receptor R2 expression and increasing c-FLIP expression.
Authors: Matte I, Lane D, Boivin M, Rancourt C, Piche A
BMC Cancer, 2014;14(0):234.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals.
Authors: Schoenmann Z, Assa-Kunik E, Tiomny S, Minis A, Haklai-Topper L, Arama E, Yaron A
J. Neurosci., 2010;30(18):6375-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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