Human MyD88 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human MyD88 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MyD88 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MyD88
    Met1-Pro296
    Accession # Q99836
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MyD88 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human MyD88 (1 ng) was included. A specific band for MyD88 was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Intracellular Staining by Flow Cytometry
Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human MyD88 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2928, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human MyD88 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 41 kDa (as indicated) using 5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MyD88
Myeloid Differentiation primary response gene 88 (MyD88) is a 35 kDa adaptor protein involved in the IL-1 signaling pathway and recruits IRAK-2 to the IL-1 receptor complex. MyD88 also recruits IRAK-4 to Toll-like receptors 2 and 4 (TLR2 and TLR4) and plays an important role in the inflammatory response induced by IL-1, IL-18 and endotoxin.
  • Long Name:
    Myeloid Differentiation Primary Response Gene 88
  • Entrez Gene IDs:
    4615 (Human); 17874 (Mouse)
  • Alternate Names:
    MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection
    PLoS Pathog, 2016;12(12):e1006102.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
    Authors: Xiang Z, Li L, Lei X, Zhou H, Zhou Z, He B, Wang J
    J Virol, 2014;88(12):6650-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Potentiation of flagellin responses in gut epithelial cells by interferon-gamma is associated with STAT-independent regulation of MyD88 expression.
    Authors: Bannon C, Davies PJ, Collett A, Warhurst G
    Biochem. J., 2009;423(1):119-28.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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