Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MyD88 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836

Specificity

Detects human MyD88 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MyD88 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MyD88 Antibody

Detection of Human MyD88 antibody by Western Blot.

Detection of Human MyD88 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human MyD88 (1 ng) was included. A specific band for MyD88 was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

MyD88 antibody in Raji Human Cell Line by Immunocytochemistry (ICC).

MyD88 in Raji Human Cell Line.

MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of MyD88 antibody in Raji Human Cell Line antibody by Flow Cytometry.

Detection of MyD88 in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of Human MyD88 antibody by Simple WesternTM.

Detection of Human MyD88 by Simple WesternTM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 41 kDa (as indicated) using 5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Shows Human MyD88 Specificity by Using Knockout Cell Line.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human MyD88 knockout Raji human Burkitt's lymphoma cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MyD88 at approximately 35 kDa (as indicated) in the parental Raji human Burkitt's lymphoma cell line, but is not detectable in knockout Raji human Burkitt's lymphoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human MyD88 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and Raji human Burkitt's lymphoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Raji human Burkitt's lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin

Knockout Validated

MyD88 is specifically detected in Raji human Burkitt's lymphoma parental cell line but is not detectable in MyD88 knockout Raji human Burkitt's lymphoma cell line.

Simple Western

5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 5 using AF2928 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MyD88

Myeloid Differentiation primary response gene 88 (MyD88) is a 35 kDa adaptor protein involved in the IL-1 signaling pathway and recruits IRAK-2 to the IL-1 receptor complex. MyD88 also recruits IRAK-4 to Toll-like receptors 2 and 4 (TLR2 and TLR4) and plays an important role in the inflammatory response induced by IL-1, IL-18 and endotoxin.

Long Name

Myeloid Differentiation Primary Response Gene 88

Alternate Names

MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88

Entrez Gene IDs

4615 (Human); 17874 (Mouse)

Gene Symbol

MYD88

UniProt

Q99836

Additional MyD88 Products

Product Documents for Human MyD88 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MyD88 Antibody

For research use only

Citations for Human MyD88 Antibody

Customer Reviews for Human MyD88 Antibody (1)

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  • Human MyD88 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 01/18/2018

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