Human MyD88 Antibody

Catalog # Availability Size / Price Qty
AF2928
AF2928-SP
Detection of Human MyD88 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human MyD88 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MyD88 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MyD88 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human MyD88
Met1-Pro296
Accession # Q99836
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below
Knockout Validated
MyD88 is specifically detected in Raji human Burkitt's lymphoma parental cell line but is not detectable in MyD88 knockout Raji human Burkitt's lymphoma cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MyD88 antibody by Western Blot. View Larger

Detection of Human MyD88 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human MyD88 (1 ng) was included. A specific band for MyD88 was detected at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry MyD88 antibody in Raji Human Cell Line by Immunocytochemistry (ICC). View Larger

MyD88 in Raji Human Cell Line. MyD88 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Intracellular Staining by Flow Cytometry Detection of MyD88 antibody in Raji Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MyD88 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human MyD88 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human MyD88 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 41 kDa (as indicated) using 5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated View Larger

Western Blot Shows Human MyD88 Specificity by Using Knockout Cell Line. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human MyD88 knockout Raji human Burkitt's lymphoma cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2928) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MyD88 at approximately 35 kDa (as indicated) in the parental Raji human Burkitt's lymphoma cell line, but is not detectable in knockout Raji human Burkitt's lymphoma cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

Myeloid Differentiation primary response gene 88 (MyD88) is a 35 kDa adaptor protein involved in the IL-1 signaling pathway and recruits IRAK-2 to the IL-1 receptor complex. MyD88 also recruits IRAK-4 to Toll-like receptors 2 and 4 (TLR2 and TLR4) and plays an important role in the inflammatory response induced by IL-1, IL-18 and endotoxin.

Long Name
Myeloid Differentiation Primary Response Gene 88
Entrez Gene IDs
4615 (Human); 17874 (Mouse)
Alternate Names
MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88

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Citations for Human MyD88 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cell-Based Screen Identifies Human Interferon-Stimulated Regulators of Listeria monocytogenes Infection
    PLoS Pathog, 2016-12-21;12(12):e1006102.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Enterovirus 68 3C protease cleaves TRIF to attenuate antiviral responses mediated by Toll-like receptor 3.
    Authors: Xiang Z, Li L, Lei X, Zhou H, Zhou Z, He B, Wang J
    J Virol, 2014-03-26;88(12):6650-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Potentiation of flagellin responses in gut epithelial cells by interferon-gamma is associated with STAT-independent regulation of MyD88 expression.
    Authors: Bannon C, Davies PJ, Collett A, Warhurst G
    Biochem. J., 2009-09-14;423(1):119-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human MyD88 Antibody
By Anonymous on 01/18/2018
Application: WB Sample Tested: Cancer cell lysates Species: Human