Human Myoglobin Antibody Summary
Accession # P02144
This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human/Mouse/Rat Myoglobin Monoclonal Antibody (Catalog # MAB97203).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Myoglobin in Human Heart. Myoglobin was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Myoglobin by Western Blot. Western blot shows lysates of human heart tissue and human liver tissue (negative control). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Myoglobin at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Human Myoglobin ELISA Standard Curve. Recombinant Human Myoglobin protein was serially diluted 2-fold and captured by Rabbit Anti-Human Myoglobin Monoclonal Antibody (Catalog # MAB97204) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human/Mouse/Rat Myoglobin Monoclonal Antibody (Catalog # MAB97203) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myoglobin is a 17KDa cytoplasmic oxygen-binding protein encoded by the MB gene and expressed in myocytes of the heart and skeletal muscle. Its name derives from its structural and functional similarity to hemoglobin, the oxygen binding protein found in red blood cells. Functions of myoglobin include oxygen storage and transport, as well as scavenging of NO and reactive oxygen species. Myoglobin also serves as a sensitive marker for muscle injury resulting from cardiac infarction. Myoglobin was the first protein to have its three-dimensional structure determined by X-ray crystallography.
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