Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 291319
View all NFkB2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF kappa B2
Met1-Asn447
Accession # Q00653

Specificity

Detects human NFkB2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3 cells is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NFkB2 Antibody

Detection of Human NF?B2 antibody by Western Blot.

Detection of Human NF kappa B2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF kappa B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of NF?B2-regulated Genes antibody by Chromatin Immunoprecipitation.

Detection of NF kappa B2-regulated Genes by Chromatin Immunoprecipitation.

Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa B2/DNA complexes were immunoprecipitated using 5 µg Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) or control antibody (Catalog # MAB002) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR.
NF?B2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

NF kappa B2 in HeLa Human Cell Line.

NF kappa B2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human NFkB2 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5 µg/5 x 106 cells
Sample: PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, c-myc promoter detected by standard PCR

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6245-CL)

Reviewed Applications

Read 6 reviews rated 4.3 using MAB28881 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NFkB2

Nuclear Factor kappa B2 (NF kappa B2 or NF kappa B p52) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B2 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Long Name

Nuclear Factor Kappa-B, Subunit 2

Alternate Names

LYT10, NF-kB2

Entrez Gene IDs

4791 (Human); 18034 (Mouse)

Gene Symbol

NFKB2

UniProt

Q00653

Additional NFkB2 Products

Product Documents for Human NFkB2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NFkB2 Antibody

For research use only

Citations for Human NFkB2 Antibody

Customer Reviews for Human NFkB2 Antibody (6)

4.3 out of 5
6 Customer Ratings
5 Stars
50%
4 Stars
33%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 6 reviews Showing All
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  • Human NFkB2 Antibody
    Name: Anonymous
    Application: Western Blot
    Verified Customer | Posted 12/31/2023
    Western Blot: whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. NFKB2 Antibody (MAB28881-100) was used for primary antibody: 1:1000, 4℃, overnight.
    Human NFkB2 Antibody MAB28881
  • Human NFkB2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human NFkB2 Antibody MAB28881
  • Human NFkB2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human NFkB2 Antibody MAB28881
  • Human NFkB2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: PDX samples
    Species: Human
    Verified Customer | Posted 01/16/2020
    Human NFkB2 Antibody MAB28881
  • Human NF kappa B2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human NF kappa B2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A375 human melanoma cell line
    Species: Human
    Verified Customer | Posted 03/01/2017
    Down-regulation of NFKB-2 p100/p52 in Human melanoma A375 cell line upon treatment with ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000.
    Human NFkB2 Antibody MAB28881

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Protocols

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