Human NFkB2 Antibody

Catalog # Availability Size / Price Qty
MAB28881-SP
MAB28881-100
Human NFkB2 Antibody in Western Blot
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Product Details
FAQs
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Reviews (4)

Human NFkB2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NFkB2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3 cells is observed.
Source
Monoclonal Mouse IgG1 Clone # 291319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human NF kappa B2
Met1-Asn447
Accession # Q00653
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human NF kappa B2 by Western Blot. View Larger

Detection of Human NF kappa B2 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF?B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF?B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of NF kappa B2-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of NF kappa B2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF?B2/DNA complexes were immunoprecipitated using 5 µg Mouse Anti-Human NF?B2 Monoclonal Antibody (Catalog # MAB28881) or control antibody (Catalog # MAB002) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thec-mycpromoter was detected by standard PCR.

Immunocytochemistry NF kappa B2 in HeLa Human Cell Line. View Larger

NF kappa B2 in HeLa Human Cell Line. NF?B2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human NF?B2 Monoclonal Antibody (Catalog # MAB28881) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NFkB2

Nuclear Factor kappa B2 (NF kappa B2 or NF kappa B p52) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B2 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Long Name
Nuclear Factor Kappa-B, Subunit 2
Entrez Gene IDs
4791 (Human); 18034 (Mouse)
Alternate Names
LYT10; NFkB2; NF-kB2

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human NFkB2 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

5 Star
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Human NFkB2 Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human NFkB2 Antibody
By Anonymous on 01/16/2020
Application: ELISA Sample Tested: PDX samples Species: Human

Human NF kappa B2 Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human NF kappa B2 Antibody
By Anonymous on 03/01/2017
Application: WB Sample Tested: A375 human melanoma cell line Species: Human

Down-regulation of NFKB-2 p100/p52 in Human melanoma A375 cell line upon treatment with ethanol. Dilution: 1:1,000 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000.