Human Nidogen-1/Entactin Antibody Summary
Accession # AAH45606.1
Human Nidogen-1/Entactin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Nidogen‑1/Entactin in Human Chondrocytes. Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.
- Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
- Miosge, N. et al. (2001) Histochem. J. 33:523.
- Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
- Timpl, R. and J.C. Brown (1996) BioEssays 18:123.
- Nagayoshi, T. et al. (1989) DNA 8:581.
- Zimmerman, K. et al. (1995) Genomics 27:245.
- Fox, J.W. et al. (1991) EMBO J. 10:3137.
- Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
- Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
- Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
- Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
- Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Citations for Human Nidogen-1/Entactin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
BMC Cancer, 2019;19(1):312.
Sample Types: Cell Lysates
Applications: Western Blot
Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
Authors: Saghizadeh M, Epifantseva I, Hemmati D, Ghiam C, Brunken W, Ljubimov A
Invest Ophthalmol Vis Sci, 2013;54(13):8172-80.
Sample Types: Whole Tissue
Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
Authors: Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
Invest. Ophthalmol. Vis. Sci., 2010;51(4):1970-80.
Sample Types: Whole Tissue
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Staining for basement membrane
Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma