Human Nidogen-1/Entactin Antibody

Catalog # Availability Size / Price Qty
MAB2570-SP
MAB2570-500
MAB2570-100
Human Nidogen-1/Entactin Antibody in Data
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (5)

Human Nidogen-1/Entactin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Nidogen‑1/Entactin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 302117
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin
Leu29-Lys1114 (Gln1113Arg)
Accession # AAH45606.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND)
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human heart
Immunocytochemistry
8-25 µg/mL
See below

Human Nidogen-1/Entactin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Nidogen-1/Entactin Biotinylated Antibody (Catalog # BAF2570)

Standard: Recombinant Human Nidogen-1/Entactin Protein, CF (Catalog # 2570-ND)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Nidogen-1/Entactin antibody in Human Chondrocytes by Immunocytochemistry (ICC). View Larger

Nidogen‑1/Entactin in Human Chondrocytes. Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.

References
  1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
  5. Nagayoshi, T. et al. (1989) DNA 8:581. 
  6. Zimmerman, K. et al. (1995) Genomics 27:245. 
  7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
  8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
  9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
  10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
  11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
  12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Entrez Gene IDs
4811 (Human)
Alternate Names
enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1

Product Datasheets

Citations for Human Nidogen-1/Entactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
    Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
    BMC Cancer, 2019;19(1):312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
    Authors: Saghizadeh M, Epifantseva I, Hemmati D, Ghiam C, Brunken W, Ljubimov A
    Invest Ophthalmol Vis Sci, 2013;54(13):8172-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
    Authors: Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
    Invest. Ophthalmol. Vis. Sci., 2010;51(4):1970-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human Nidogen-1/Entactin Antibody

Average Rating: 3.6 (Based on 5 Reviews)

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Human Nidogen-1/Entactin Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Nidogen-1/Entactin Antibody
By Anonymous on 12/02/2020
Application: ICC/IF Sample Tested: Differentiated embryonic stem cells Species: Human

Staining for basement membrane


Human Nidogen-1/Entactin Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human Nidogen-1/Entactin Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Nidogen-1/Entactin Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human