Human Nidogen-1/Entactin Antibody

Catalog # Availability Size / Price Qty
Nidogen‑1/Entactin in Human Chondrocytes.
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Product Details
Citations (3)
Supplemental Products
Reviews (7)

Human Nidogen-1/Entactin Antibody Summary

Species Reactivity
Detects human Nidogen‑1/Entactin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.
Monoclonal Mouse IgG1 Clone # 302117
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Nidogen‑1/Entactin
Leu29-Lys1114 (Gln1113Arg)
Accession # AAH45606.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Nidogen‑1/Entactin (Catalog # 2570-ND)
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human heart
8-25 µg/mL
See below

Human Nidogen-1/Entactin Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Nidogen-1/Entactin Biotinylated Antibody (Catalog # BAF2570)

Standard: Recombinant Human Nidogen-1/Entactin Protein, CF (Catalog # 2570-ND)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Nidogen-1/Entactin antibody in Human Chondrocytes by Immunocytochemistry (ICC). View Larger

Nidogen‑1/Entactin in Human Chondrocytes. Nidogen-1/Entactin was detected in immersion fixed human mesenchymal stem cells differentiated into chondrocytes using Mouse Anti-Human Nidogen-1/Entactin Monoclonal Antibody (Catalog # MAB2570) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nidogen-1/Entactin

Nidogen-1 (also entactin) is a 150 kDa, secreted, monomeric glycoprotein that serves as a major linking component of basement membranes (1-4). It is synthesized as a 1247 amino acid (aa) precursor with a 28 aa signal sequence and a 1219 aa mature protein. The molecule is modular in structure with five distinct regions. There are three globular domains (G1-3) separated by a mucin region and an extended rod-shaped segment (5-7). The N-terminal globular domain (G1) is 200 aa in length and seemingly unrelated to any known motif (8). The mucin region is nearly 160 aa in length and presumably O-glycosylated (2, 8). G2 and G3 are both approximately 300 aa in length. G2 is described as a Nidogen ( beta -barrel) domain, while C-terminal G3 assumes a beta -propeller configuration (1). The 250 aa rod-shaped segment has multiple EGF-like motifs and two thyroglobulin type 1 domains. Functionally, G1 is reported to bind type IV collagen (2, 7). The mucin region contains a short peptide that ligates alpha 3 beta 1 integrins (9, 10). G2 interacts with perlecan, and an RGD motif in the rod-shaped segment serves as a binding site for alpha v beta 3 integrins (9, 10). Finally, G3 is associated with laminin binding (2, 7). As a full-length molecule, the multiple extracellular matrix-binding sites of Nidogen-1 are well positioned to serve as anchor sites for basement membrane molecules. Nidogen-1 also undergoes proteolytic processing by at least two MMPs, MMP-7 and MMP-19 (10, 11). While this destroys the integrity of Nidogen-associated matrices, it also generates peptide fragments that are capable of inducing neutrophil chemotaxis and phagocytosis (10). Nidogen-2 is related to Nidogen-1 (≈ 50% aa identity) and shares many of the same adhesive properties as Nidogen-1 (12). Both bind perlecan plus collagens I and IV. Nidogen‑2, however, does not bind fibulin-1 or 2, and shows only modest interaction with laminin. Thus, although coexpressed, Nidogen-2 serves as only a partial substitute for Nidogen-1 (2, 12). Human Nidogen-1 shares 85% aa sequence identity with both mouse and rat Nidogen-1, and 88% aa sequence identity with canine Nidogen-1.

  1. Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115. 
  2. Miosge, N. et al. (2001) Histochem. J. 33:523.
  3. Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
  4. Timpl, R. and J.C. Brown (1996) BioEssays 18:123. 
  5. Nagayoshi, T. et al. (1989) DNA 8:581. 
  6. Zimmerman, K. et al. (1995) Genomics 27:245. 
  7. Fox, J.W. et al. (1991) EMBO J. 10:3137.
  8. Mayer, U. et al. (1995) Eur. J. Biochem. 227:681.
  9. Gresham, H.D. et al. (1996) J. Biol. Chem. 271:30587.
  10. Dong, L-J. et al. (1995) J. Biol. Chem. 270:15383.
  11. Titz, B. et al. (2004) Cell. Mol. Life Sci. 61:1826.
  12. Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
Entrez Gene IDs
4811 (Human)
Alternate Names
enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1

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Citations for Human Nidogen-1/Entactin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Endothelial cell-derived nidogen-1 inhibits migration of SK-BR-3 breast cancer cells
    Authors: DA Ferraro, F Patella, S Zanivan, C Donato, N Aceto, M Giannotta, E Dejana, M Diepenbruc, G Christofor, M Buess
    BMC Cancer, 2019;19(1):312.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.
    Authors: Saghizadeh M, Epifantseva I, Hemmati D, Ghiam C, Brunken W, Ljubimov A
    Invest Ophthalmol Vis Sci, 2013;54(13):8172-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Normalization of wound healing and diabetic markers in organ cultured human diabetic corneas by adenoviral delivery of c-Met gene.
    Authors: Saghizadeh M, Kramerov AA, Yu FS, Castro MG, Ljubimov AV
    Invest. Ophthalmol. Vis. Sci., 2010;51(4):1970-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC


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Reviews for Human Nidogen-1/Entactin Antibody

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Human Nidogen-1/Entactin Antibody
By Ruchi Shah on 03/21/2023
Application: IHC Sample Tested: corneal tissue Species: Human

1% formalin fixed

Human Nidogen-1/Entactin Antibody
By Anonymous on 11/01/2021
Application: ICC/IF Sample Tested: Lung tissue Species: Human

Human Nidogen-1/Entactin Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Nidogen-1/Entactin Antibody
By Pinyuan Tian on 12/02/2020
Application: ICC/IF Sample Tested: Differentiated embryonic stem cells Species: Human

Staining for basement membrane

Human Nidogen-1/Entactin Antibody
By Anonymous on 02/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Human Nidogen-1/Entactin Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Nidogen-1/Entactin Antibody
By Ed Lo on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human