Human NKp46/NCR1 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKp46 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse NKp46 (MAR-1) is observed and less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp44, and rhNKp80 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human NKp46/NCR1
    Gln22-Asn254
    Accession # AAH64806
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
  • Immunohistochemistry
    3-25 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
NKp46/NCR1 in NK‑92 human cell line NKp46/NCR1 was detected in immersion fixed
NK-92 human natural killer lymphoma cell line using Goat Anti-Human NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1850) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
NKp46/NCR1 in Human Lymph Node. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1850) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp46/NCR1

NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with the
ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5-7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

  • References:
    1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255. 
    2. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
    3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235. 
    4. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
    5. Arnon, T. et al. (2004) Blood 103:664.
    6. Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
    7. Mandelboim, O. et al. (2001) Nature 409:1055.
    8. Moretta, A. et al. (2001) Annu. Rev. Immunol. 19:197.
  • Entrez Gene IDs:
    9437 (Human); 17086 (Mouse)
  • Alternate Names:
    CD335 antigen; CD335; hNKp46; Ly94; LY94lymphocyte antigen 94 homolog (activating NK-receptor; NK-p46); Lymphocyte antigen 94 homolog; MAR-1; natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NK cell-activating receptor; NKp46; NKP46FLJ99094; NK-p46lymphocyte antigen 94 (mouse) homolog (activating NK-receptor; NK-p46)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
    Mucosal Immunol, 2016;9(6):1584-1595.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Evidence of innate lymphoid cell redundancy in humans
    Authors: F Vély, V Barlogis, B Vallentin, B Neven, C Piperoglou, M Ebbo, T Perchet, M Petit, N Yessaad, F Touzot, J Bruneau, N Mahlaoui, N Zucchini, C Farnarier, G Michel, D Moshous, S Blanche, A Dujardin, H Spits, JH Distler, A Ramming, C Picard, R Golub, A Fischer, E Vivier
    Nat. Immunol, 2016;17(11):1291-1299.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014;9(8):e105375.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion.
    Authors: Peppa, Dimitra, Gill, Upkar S, Reynolds, Gary, Easom, Nicholas, Pallett, Laura J, Schurich, Anna, Micco, Lorenzo, Nebbia, Gaia, Singh, Harsimra, Adams, David H, Kennedy, Patrick, Maini, Mala K
    J Exp Med, 2013;210(1):99-114.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
    Authors: Durrenberger PF, Ettorre A, Kamel F
    J Neuroinflammation, 2012;9(0):1.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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