Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 257219
View all Olig1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Olig1

Specificity

Detects human Olig1. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Olig1 Antibody

Olig1 antibody in Human Glioma by Immunohistochemistry (IHC-P).

Olig1 in Human Glioma.

Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Olig1 antibody by Western Blot.

Detection of Human Olig1 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Olig1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human glioma

Western Blot

0.5 µg/mL
Sample: SH-SY5Y human neuroblastoma cell line

Reviewed Applications

Read 2 reviews rated 3 using MAB2417 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Olig1

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of progenitors of embryonic central nervous system and then disappears in the course of development. In adult, expression of Olig3 is primarily detected in neural tissues.

Long Name

Oligodendrocyte Lineage Gene 1

Alternate Names

BHLHB6

Entrez Gene IDs

116448 (Human)

Gene Symbol

OLIG1

Additional Olig1 Products

Product Documents for Human Olig1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Olig1 Antibody

For research use only

Citations for Human Olig1 Antibody

Customer Reviews for Human Olig1 Antibody (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
50%

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Customer Images

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  • Human/Mouse Olig1 Antibody
    Name: Claudia Belem
    Application: Immunohistochemistry
    Sample Tested: Embryonic spinal cord
    Species: Mouse
    Verified Customer | Posted 03/13/2024
    Section co-stained with anti-Olig1 (in green), anti-Olig2 (in magenta) and anti-Pdgfra(not shown). I was expecting some degree of co-localisation in Olig1 and Olig2. Olig1 seems to be non specific and not to have worked.
    Human Olig1 Antibody MAB2417
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  • Human/Mouse Olig1 Antibody
    Name: safa azar
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain (cerebral cortex) and Cancer Tissue
    Species: Human
    Verified Customer | Posted 06/08/2018
    Human Olig1 Antibody MAB2417

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Protocols

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FAQs

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