Human Orexin B/Hypocretin-2 Antibody

Catalog # Availability Size / Price Qty
MAB734
MAB734-SP
Orexin B in Mouse Hypothalamus.
2 Images
Product Details
Citations (1)
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Human Orexin B/Hypocretin-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.
Source
Monoclonal Mouse IgG1 Clone # 145202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Orexin B antibody in Mouse Hypothalamus by Immunohistochemistry (IHC-Fr). View Larger

Orexin B in Mouse Hypothalamus. Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr). View Larger

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Orexin B/Hypocretin-2

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein (Accession # O43612). Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism.

Entrez Gene IDs
3060 (Human)
Alternate Names
Hcrt 2; HCRT; Hcrt2; Hypocretin (orexin) neuropeptide precursor; Hypocretin 2; Hypocretin B; Hypocretin precursor; Hypocretin; Hypocretin2; Hypocretin-2; Orexin B; Orexin neuropeptide precursor; Orexin precursor; Orexin; OrexinB; PPORX; PPOX

Product Datasheets

Citation for Human Orexin B/Hypocretin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Localization of orexin B and receptor 2 for orexins in testicular cytotypes of the camelid alpaca (Vicugna pacos)
    Authors: G Liguori, C Squillacio, L Assisi, N Mirabella, E Langella, A Costagliol, A Vittoria
    Reprod. Domest. Anim, 2017;0(0):.
    Species: Alpaca
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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