Key Product Details

Species Reactivity

Validated:

Human

Cited:

Camelid - Vicugna pacos (Alpaca), Canine

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 145202
View all Orexin B/Hypocretin-2 Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612

Specificity

Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Orexin B/Hypocretin‑2 Antibody

Orexin B antibody in Mouse Hypothalamus by Immunohistochemistry (IHC-Fr).

Orexin B in Mouse Hypothalamus.

Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr).

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.

Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Anti-Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Porcine Orexin B/Hypocretin-2 by Immunohistochemistry

Detection of Porcine Orexin B/Hypocretin-2 by Immunohistochemistry

(A) Cytoplasmatic positivity for orexin B in granulosa cells (20X IHC). Scale bar = 100 µm. (B) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34204547), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Porcine Orexin B/Hypocretin-2 by Immunohistochemistry

Detection of Porcine Orexin B/Hypocretin-2 by Immunohistochemistry

(A) Cytoplasmatic positivity for orexin B in granulosa cells (20X IHC). Scale bar = 100 µm. (B) Negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34204547), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Orexin B/Hypocretin‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem and hypothalamus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Orexin B/Hypocretin-2

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein (Accession # O43612). Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism.

Alternate Names

Hcrt2, Hypocretin-2, Hypocretin2, PPORX, PPOX

Entrez Gene IDs

3060 (Human)

Gene Symbol

HCRT

UniProt

O43612

Additional Orexin B/Hypocretin-2 Products

Product Documents for Human Orexin B/Hypocretin‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Orexin B/Hypocretin‑2 Antibody

For research use only

Citations for Human Orexin B/Hypocretin‑2 Antibody

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Protocols

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