Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p38 alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p38 alpha
Met1-Ser360
Accession # Q16539

Specificity

Detects human, mouse and rat p38 alpha. Does not detect recombinant human p38 beta, p38 gamma or p38 delta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat p38 alpha Antibody

Detection of Human, Mouse, and Rat p38a antibody by Western Blot.

Detection of Human, Mouse, and Rat p38 alpha by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band for p38a was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human p38a antibody by Western Blot.

Detection of Human p38 alpha by Western Blot.

Western blot shows recombinant human p38 beta, p38 gamma, p38 delta and Recombinant Human Active p38 alpha (5477-KS) (2 ng/lane). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr).

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem.

Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; MAB734), Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Human and Mouse p38a antibody by Simple WesternTM.

Detection of Human and Mouse p38 alpha by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for p38 alpha at approximately 43 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human p38 alpha Specificity by Using Knockout Cell Line.

Western Blot Shows Human p38 alpha Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and p38a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for p38a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of p38 alpha in Mouse Brain Cerebellum.

p38 alpha was detected in perfusion fixed paraffin-embedded sections of Mouse Brain Cerebellum using Rabbit Anti-Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8691) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human p38 alpha by Western Blot

Detection of Human p38 alpha by Western Blot

Pharmacological and genetic inhibition of the p38 MAPK pathway and its impact on human myotube formation in vitro.(A) Luminometric analysis of cell lysates generated from co-cultures initiated with 105 myoblastFLPe and 105 myoblastGS.Luc cells and exposed for 3 days to differentiation medium (white bar), to differentiation medium supplemented with 0.1, 0.5 and 2.5 µM SB 203580 (black bars) or to differentiation medium containing a final concentration of vehicle equivalent to that applied to co-cultures incubated with 2.5 µM SB 203580 (grey bar). Cumulative data are presented as means ± standard deviations (n = 3). RLU, relative light units. (B) Western blot analysis of p38 alpha levels in protein lysates of parental myoblasts and myoblastsGS.Luc (mock) and of myoblasts and myoblastsGS.Luc stably transduced with shRNA modules designed to down-regulate expression of eGFP (sh.eGFP), hif1 alpha (sh.hif1 alpha ) and human p38 alpha (sh.p38 alpha.35, sh.p38 alpha.33, sh.p38 alpha.36 and sh.p38 alpha.34). The alpha - and beta -tubulins served as loading control. (C) Diagram outlining the experimental set-up applied to investigate the impact of post-transcriptional down-regulation of p38 alpha expression on human myocyte fusion (see text for details). (D) Quantification through chemiluminescence of myoblast fusion activity in co-cultures consisting of a 1∶1 mixture of FLPe- and GS.Luc-encoding myoblasts either not transduced (none) or stably transduced with shRNAs sh.p38 alpha.33, sh.p38 alpha.36 or sh.hif1 alpha. Data were derived from a minimum of 3 and a maximum of 6 different experiments and are presented as means ± standard error of the mean. RLU, relative light units. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20532169), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat p38 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brainstem and perfusion fixed paraffin-embedded sections of Mouse Brain Cerebellum

Knockout Validated

p38 alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in p38 alpha knockout HEK293T cell line.

Simple Western

5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line

Reviewed Applications

Read 3 reviews rated 5 using AF8691 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Alternate Names

MAPK14, SAPK2a

Entrez Gene IDs

1432 (Human); 26416 (Mouse); 81649 (Rat)

Gene Symbol

MAPK14

UniProt

Q16539

Additional p38 alpha Products

Product Documents for Human/Mouse/Rat p38 alpha Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p38 alpha Antibody

For research use only

Citations for Human/Mouse/Rat p38 alpha Antibody

Customer Reviews for Human/Mouse/Rat p38 alpha Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 05/17/2022
    Very good detection peaks while using Simple Western, as indicated.
    Human/Mouse/Rat p38 alpha Antibody AF8691
  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human breast cancer cells
    Species: Human
    Verified Customer | Posted 10/24/2018
    Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by western blot.
    Human/Mouse/Rat p38 alpha Antibody AF8691
  • Human/Mouse/Rat p38 alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/17/2018
    Human/Mouse/Rat p38 alpha Antibody AF8691

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