Mouse/Rat Integrin  beta 1/CD29 Antibody

(6 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1
    Gln21-Ala738
    Accession # P09055
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    B16‑F10 mouse melanoma cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Integrin  beta 1/CD29 by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑3 human prostate cancer cell line, Rat‑2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin  beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38 alpha, and Integrin  beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38 alpha Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin  beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat Integrin  beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of L‑929 mouse fibroblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, PC‑12 rat adrenal pheochromocytoma cell line, and Rat‑2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin  beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.
  • Entrez Gene IDs:
    3688 (Human); 16412 (Mouse)
  • Alternate Names:
    CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
    Authors: Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L
    Gene Ther, 2014;21(9):785-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  3. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011;118(4):521-32.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  4. Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
    Authors: Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
    Arthritis Rheum., 2009;60(9):2817-21.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Galectin-7 in the control of epidermal homeostasis after injury.
    Authors: Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
    Mol. Biol. Cell, 2008;19(12):5541-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007;120(0):3053-63.
    Species: Rat
    Sample Type: Cell Lysates
    Application: IP
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