Mouse/Rat Integrin  beta 1/CD29 Antibody

Catalog # Availability Size / Price Qty
AF2405
AF2405-SP
Detection of Mouse and Rat Integrin  beta 1/CD29 by Western Blot.
3 Images
Product Details
Citations (8)
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Mouse/Rat Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse Integrin beta 1/CD29 in direct ELISAs. Detects mosue and rat Integrin beta 1/CD29 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Integrin beta 1 and rhIntegrin beta 3 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin beta 2, rmIntegrin beta 4, and rmIntegrin beta 6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1
Gln21-Ala738
Accession # P09055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
B16‑F10 mouse melanoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Integrin  beta 1/CD29 antibody by Western Blot. View Larger

Detection of Mouse and Rat Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-3 human prostate cancer cell line, Rat-2 rat embryonic fibroblast cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin beta 1/CD29 at approximately 110-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Orexin B, p38a, & Integrin beta 1 antibody in Mouse Brainstem by Immunohistochemistry (IHC-Fr). View Larger

Orexin B, p38 alpha, & Integrin beta 1 in Mouse Brainstem. Orexin B, p38a, and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Human/Mouse/Rat p38a Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF8691), and Mouse/Rat Integrin beta 1 Antigen Affinity-purified Polyclonal Antibody (blue; Catalog # AF2405). Tissue was incubated with primary antibodies overnight at 4 °C. Tissue was stained using NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # NL006), NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007), and NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (blue; Catalog # NL002). The image of Integrin beta 1 is pseudo-colored for presentation. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse and Rat Integrin  beta 1/CD29 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse and Rat Integrin beta 1/CD29 by Simple WesternTM. Simple Western lane view shows lysates of L-929 mouse fibroblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin beta 1/CD29 at approximately 153-174 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2405) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Citations for Mouse/Rat Integrin  beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis
    Authors: A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco
    Cell Reports, 2021;37(4):109885.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis
    Authors: A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco
    Cell Rep, 2020;31(2):107513.
    Species: Mouse
    Sample Types: Testes
    Applications: IF
  3. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko mice.
    Authors: Wu, B, Cloer, C, Lu, P, Milazi, S, Shaban, M, Shah, S N, Marston-Poe, L, Moulton, H M, Lu, Q L
    Gene Ther, 2014;21(9):785-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011;118(4):521-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  6. Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse model.
    Authors: Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A
    Arthritis Rheum., 2009;60(9):2817-21.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Galectin-7 in the control of epidermal homeostasis after injury.
    Authors: Gendronneau G, Sidhu SS, Delacour D, Dang T, Calonne C, Houzelstein D, Magnaldo T, Poirier F
    Mol. Biol. Cell, 2008;19(12):5541-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Tetraspanin CD81 is required for the alpha v beta5-integrin-dependent particle-binding step of RPE phagocytosis.
    Authors: Chang Y, Finnemann SC
    J. Cell. Sci., 2007;120(0):3053-63.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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