Human Pax2 Antibody Summary
Asp229-Pro363
Accession # Q02962
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of Mouse Pax2 by Western Blot. Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Pax2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Pax2 in BG01V Human Embyonic Stem Cells. Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Pax2 in Human Kidney. Pax2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax2
Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.
Product Datasheets
Citations for Human Pax2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain
Authors: F Aby, LE Lorenzo, Z Grivet, R Bouali-Ben, H Martin, S Valerio, S Whitestone, D Isabel, W Idi, O Bouchatta, P De Deurwae, AG Godin, C Herry, X Fioramonti, M Landry, Y De Koninck, P Fossat
Science Advances, 2022;8(30):eabo0689.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A gastruloid model of the interaction between embryonic and extra-embryonic cell types
Authors: NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau
Journal of tissue engineering, 2022;13(0):2041731422110.
Species: Mouse
Sample Types: Organoids
Applications: IHC -
Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
Authors: RR Das Gupta, L Scheurer, P Pelczar, H Wildner, HU Zeilhofer
Scientific Reports, 2021;11(1):5232.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer
Authors: EA Paris, JM Bahr, P Bitterman, S Basu, JS Abramowicz, A Barua
PLoS ONE, 2021;16(7):e0255007.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
Cell Reports, 2021;37(10):110095.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
Cell Rep, 2020;33(8):108425.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Spinal Inhibitory Ptf1a-Derived Neurons Prevent Self-Generated Itch
Authors: A Escalante, R Klein
Cell Rep, 2020;33(8):108422.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Targeting spinal neuropeptide Y1 receptor-expressing interneurons to alleviate chronic pain and itch
Authors: TS Nelson, BK Taylor
Prog. Neurobiol., 2020;0(0):101894.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn
Authors: TS Nelson, W Fu, RR Donahue, GF Corder, T Hökfelt, RG Wiley, BK Taylor
Sci Rep, 2019;9(1):7248.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis
Authors: IS Zaitoun, CM Wintheiser, N Jamali, S Wang, A Suscha, SR Darjatmoko, K Schleck, BA Hanna, V Lindner, N Sheibani, CM Sorenson
Sci Rep, 2019;9(1):9700.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Histone deacetylase expression patterns in developing murine optic nerve.
Authors: Tiwari, Sarika, Dharmarajan, Subraman, Shivanna, Mahesh, Otteson, Deborah, Belecky-Adams, Teri L
BMC Dev Biol, 2014;14(0):30.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis.
Authors: Zaitoun I, Wintheiser C, Jamali N, Wang S, Suscha A, Darjatmoko S, Schleck K, Hanna B, Lindner V, Sheibani N, Sorenson C
Sci Rep, 0;9(1):9700.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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