Human Pax2 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Pax2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax3, rhPax4, rhPax5, rhPax6, and rhPax7 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human Pax2
  Asp229-Pro363
  Accession # Q02962
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Immunocytochemistry
  5-15 µg/mL
  See below

Western Blot click image to view larger

Detection of Human Pax2 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pax2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry click image to view larger

Pax2 in BG01V Human Embyonic Stem Cells. Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Paired Box Gene 2

• Entrez Gene IDs:
  5076 (Human); 18504 (Mouse); 293992 (Rat)

• Alternate Names:
  paired box 2; paired box gene 2; paired box homeotic gene 2; paired box protein Pax-2; Pax2

FAQs

No product specific FAQs exist for this product, however you may