Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pax2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax2
Asp229-Pro363
Accession # Q02962

Specificity

Detects human Pax2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pax2 Antibody

Detection of Mouse Pax2 by Western Blot.

Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Pax2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Pax2 antibody in BG01V Human Embyonic Stem Cells by Immunocytochemistry (ICC).

Pax2 in BG01V Human Embyonic Stem Cells.

Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Pax2 in Human Kidney.

Pax2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Ablation of AAV-PdynP+ spinal dorsal horn (SDH) neurons does not affect behavioral responses evoked by the optical stimulation of A beta fibers, nociceptive mechanical force, and heat in rats. (A) Enhanced green fluorescent protein (EGFP) expression (green) in the L4 SDH at 4 weeks after intra-SDH microinjection of AAV-PdynP-DTR-EGFP (indicated by an upper schematic illustration). Colocalization of EGFP and PAX2 (red) in the SDH (indicated by arrowheads). Scale bar, 100 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 94 total EGFP+ cells quantified, and 339 total PAX2+ cells quantified). (B) Diphtheria toxin receptors (DTR) immunofluorescence in the SDH after administration of diphtheria toxin (DTX: 50 μg/kg, i.p., two injection 72 h apart) or PBS. Scale bar, 200 μm. (C) Immunohistochemical analysis and quantification of PAX2+ neurons in the superficial laminae of the ipsilateral and contralateral SDH at 4 weeks after DTX injection (n = 6 rats tested, PAX2+ neurons: 992 [ipsi] and 1574 [contra] cells quantified). Scale bar, 50 μm. ****P < 0.0001, Unpaired t-test. (D) Paw withdrawal behaviors by light (score), von Frey filaments (threshold), and heat (latency) at 4 weeks after DTX or PBS injection (n = 7–9 rats). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Human Pax2 Antibody by Immunohistochemistry

Detection of Rat Human Pax2 Antibody by Immunohistochemistry

AAV-PdynP injected into rat spinal dorsal horn (SDH) captures a subset of inhibitory neurons. (A) tdTomato (tdT) expression in the fourth lumbar (L4) SDH at 4 weeks after microinjection of AAV-PdynP-tdT. Schematic illustration indicates intra-SDH microinjection of the AAV vector. Scale bar, 200 μm. (B) Immunolabeling of tdT+ cells (red) with lamina-selective markers (green, NK1R; blue, IB4) in the L4 SDH. Scale bar, 200 μm. Quantification of the distribution of tdT+ cells (n = 3 rats tested, 272 total tdT+ cells quantified). (C) Immunofluorescence of prodynorphin (PDYN; green) in tdT+ SDH neurons (red). Scale bar, 200 μm (above), 20 μm (below). Arrowheads indicate tdT+ PDYN+ neurons. The percentage of tdT+ PDYN+ neurons per total tdT+ neurons was quantified (n = 4 rats tested, 1,281 total tdT+ cells quantified). (D) No colocalization of tdT (red) with NK1R (green). Scale bar, 50 μm. The percentage of co-expressing neurons was quantified (n = 3 rats tested, 187 total tdT+ cells quantified). (E) Firing patterns of tdT+ SDH neurons, and the percentage of neurons displaying each pattern. APs were evoked by current injection (80 pA). (F) Colocalization of tdT (red) with PAX2 (blue) and VGLUT2 (green) in the superficial laminae. Arrowheads and arrows indicate tdT+PAX2+ and tdT+VGLUT2+ neurons, respectively. Scale bar, 50 μm. The percentage of co-expressing neurons was quantified (n = 4 rats tested, 456 total tdT+ cells quantified). Data shown as mean ± SEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35813063), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Pax2 by Immunohistochemistry

Detection of Mouse Pax2 by Immunohistochemistry

MiR34/449 tunes optimal Satb2 expression in the Ctip2 IN subpopulation.(A) Immunostaining for Satb2, Ctip2,&Pax2 in dorsal&intermediate spinal cord at P14. Note that Satb2on INs display two molecular identities, i.e., the Ctip2on or Pax2on subpopulations. Comparison with WT/Ctrl reveals an increase in the cell number of Satb2on;Ctip2on INs in the intermediate spinal cord of cervical/brachial sections upon MiR34/449 deletion. Boxed areas indicate higher magnification regions from the intermediate&dorsal regions of each image. Arrows highlight the presence of dual-labeled Satb2on;Ctip2on cells (yellow) or dual-labeled Satb2on;Pax2on cells (magenta) among the Satb2on population. (B–C) Quantifications of cell number from (A). (D) Quantifications of the Satb2on;Ctip2on&Satb2on;Pax2on subpopulations normalized against total Satb2on cell numbers&are shown as a %age. (E) Comparison of the spatial distribution of individual Satb2on;Ctip2on INs in the cervical/brachial spinal cord of indicated mice (Ctrl, grey; Mir34bc/449 DKO&Mir34/449 TKO, magenta). Frequency distributions along the medio-lateral (top)&dorso-ventral (right) axes represent non-linear regressions. (F) A contour density plot of Satb2on;Ctip2on INs indicates that Satb2on;Ctip2on INs are mainly located in the medial spinal cord of both MiR34/449 mutant&Ctrl mice. Contour lines represent density at the 30th–90th %iles. Data analyzed from more than 25 sections of at least five independent spinal cords in (E)&(F). (G) Schematic illustrating the increased number of Satb2on;Ctip2on INs in the MiR34/449 mutant spinal cord. Data are presented as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test. * denotes p<0.05; ‘NS’ denotes ‘not significant’. Scale bars represent 100 μm. Image collected&cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33787491), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Pax2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line and BG01V human embryonic stem cells differentiated into early otic lineage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

1 µg/mL
Sample: Mouse fetal kidney

Reviewed Applications

Read 2 reviews rated 4 using AF3364 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pax2

Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.

Long Name

Paired Box Gene 2

Alternate Names

paired box 2, paired box gene 2, paired box homeotic gene 2, paired box protein Pax-2

Entrez Gene IDs

5076 (Human); 18504 (Mouse); 293992 (Rat)

Gene Symbol

PAX2

UniProt

Q02962

Additional Pax2 Products

Product Documents for Human Pax2 Antibody

Certificate of Analysis

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Product Specific Notices for Human Pax2 Antibody

For research use only

Citations for Human Pax2 Antibody

Customer Reviews for Human Pax2 Antibody (2)

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  • Human Pax2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS2 human induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 03/27/2024
    Green is PAX2. Blue is DAPI.
    Human Pax2 Antibody AF3364
  • Human Pax2 Antibody
    Name: Juan Zanin
    Application: Immunohistochemistry
    Sample Tested: Cerebellum tissue
    Species: Mouse
    Verified Customer | Posted 07/22/2016
    P7 Mouse cerebellum. 20 um section.
    Human Pax2 Antibody AF3364

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