Detection of Human Pax2 by Western Blot.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pax2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Pax2 in BG01V Human Embyonic Stem Cells.
Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.
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