Human Pax2 Antibody

Catalog # Availability Size / Price Qty
AF3364
AF3364-SP
Detection of Mouse Pax2 by Western Blot.
3 Images
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Human Pax2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pax2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Pax2
Asp229-Pro363
Accession # Q02962
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human kidney
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse Pax2 by Western Blot. Western blot shows lysates of mouse fetal kidney. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Pax2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry Pax2 antibody in BG01V Human Embyonic Stem Cells by Immunocytochemistry (ICC). View Larger

Pax2 in BG01V Human Embyonic Stem Cells. Pax2 was detected in immersion fixed BG01V human embryonic stem cells differentiated into the early otic lineage using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunohistochemistry View Larger

Pax2 in Human Kidney. Pax2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Pax2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3364) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pax2

Pax2 is a 40-45 kDa protein belonging to the small but developmentally important family of transcription regulators. Human Pax2 is a 416 amino acid (aa) residue protein with an N-terminal 128 aa DNA-binding paired box domain, a centrally-located octapeptide motif and a C-terminal truncated homeodomain. Based on the presence of the structural domains, Pax2 belongs to subgroup 2 in the Pax family. Pax2 is important for stem cell survival and lineage commitment during development. Pax2 is also expressed in various carcinomas where it seems to mediate anti-apoptotic functions. At least five splice isoforms of human Pax2 have been described within the region used as immunogen (shared by all isoforms). Human and mouse Pax2 share 98% aa sequence identity.

Long Name
Paired Box Gene 2
Entrez Gene IDs
5076 (Human); 18504 (Mouse); 293992 (Rat)
Alternate Names
paired box 2; paired box gene 2; paired box homeotic gene 2; paired box protein Pax-2; Pax2

Product Datasheets

You must select a language.

x

Citations for Human Pax2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain
    Authors: F Aby, LE Lorenzo, Z Grivet, R Bouali-Ben, H Martin, S Valerio, S Whitestone, D Isabel, W Idi, O Bouchatta, P De Deurwae, AG Godin, C Herry, X Fioramonti, M Landry, Y De Koninck, P Fossat
    Science Advances, 2022;8(30):eabo0689.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A gastruloid model of the interaction between embryonic and extra-embryonic cell types
    Authors: NM Bérenger-C, M Mircea, E Adegeest, PR van den Be, M Feliksik, M Hochane, T Idema, SJ Tans, S Semrau
    Journal of tissue engineering, 2022;13(0):2041731422110.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  3. Neuron-specific spinal cord translatomes reveal a neuropeptide code for mouse dorsal horn excitatory neurons
    Authors: RR Das Gupta, L Scheurer, P Pelczar, H Wildner, HU Zeilhofer
    Scientific Reports, 2021;11(1):5232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Incidence of malignant transformation in the oviductal fimbria in laying hens, a preclinical model of spontaneous ovarian cancer
    Authors: EA Paris, JM Bahr, P Bitterman, S Basu, JS Abramowicz, A Barua
    PLoS ONE, 2021;16(7):e0255007.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation
    Authors: Y Lan, KM Banks, H Pan, N Verma, GR Dixon, T Zhou, B Ding, O Elemento, S Chen, D Huangfu, T Evans
    Cell Reports, 2021;37(10):110095.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Spinal Inhibitory Ptf1a-Derived Neurons Prevent Self-Generated Itch
    Authors: A Escalante, R Klein
    Cell Rep, 2020;33(8):108422.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Targeting spinal neuropeptide Y1 receptor-expressing interneurons to alleviate chronic pain and itch
    Authors: TS Nelson, BK Taylor
    Prog. Neurobiol., 2020;0(0):101894.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Facilitation of neuropathic pain by the NPY Y1 receptor-expressing subpopulation of excitatory interneurons in the dorsal horn
    Authors: TS Nelson, W Fu, RR Donahue, GF Corder, T Hökfelt, RG Wiley, BK Taylor
    Sci Rep, 2019;9(1):7248.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis
    Authors: IS Zaitoun, CM Wintheiser, N Jamali, S Wang, A Suscha, SR Darjatmoko, K Schleck, BA Hanna, V Lindner, N Sheibani, CM Sorenson
    Sci Rep, 2019;9(1):9700.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Histone deacetylase expression patterns in developing murine optic nerve.
    Authors: Tiwari, Sarika, Dharmarajan, Subraman, Shivanna, Mahesh, Otteson, Deborah, Belecky-Adams, Teri L
    BMC Dev Biol, 2014;14(0):30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Bcl-2 Expression in Pericytes and Astrocytes Impacts Vascular Development and Homeostasis.
    Authors: Zaitoun I, Wintheiser C, Jamali N, Wang S, Suscha A, Darjatmoko S, Schleck K, Hanna B, Lindner V, Sheibani N, Sorenson C
    Sci Rep, 0;9(1):9700.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Pax2 Antibody

Average Rating: 4 (Based on 1 Review)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Pax2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Pax2 Antibody
By Juan Zanin on 07/22/2016
Application: IHC Sample Tested: Cerebellum tissue Species: Mouse

P7 Mouse cerebellum. 20 um section.