Human PDGF R beta Antibody

Name: Human PDGF R beta Antibody
Brand: R&D Systems

(16 citations)

Datasheet / CoA / SDS

PDGF R beta  in Human Breast Cancer Tissue.

Immunohistochemistry

View Details

Neutralization

View Details

Product Details

Citations (16)

FAQs

Supplemental Products

Reviews

Species Reactivity

Human
Specificity

Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PDGF R beta is observed, and less than 1% cross-reactivity with recombinant human PDGF sR alpha is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
Leu33-Phe530 (Glu241Asp)
Accession # P09619
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)
Flow Cytometry

2.5 µg/10⁶ cells

BUD‑8 human fibroblast cell line
Immunohistochemistry

5-15 µg/mL

See below
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize PDGF R beta inhibition of PDGF-BB-dependent proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 10-40 µg/mL in the presence of 2 μg/mL Recombinant Human PDGF R beta Fc Chimera and 4 ng/mL Recombinant Human PDGF-BB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry

click image to view larger

PDGF R beta in Human Breast Cancer Tissue.

PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

click image to view larger

PDGF R beta Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody.

Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220‑BB) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND₅₀ is typically
10‑40 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

References:
1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Long Name:

Platelet-derived Growth Factor Receptor beta

Entrez Gene IDs:

5159 (Human); 18596 (Mouse); 24629 (Rat)

Alternate Names:

beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Species	Applications	Sample Type
Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro PLoS ONE, 2017;12(8):e0182265. Species: Human Sample Type: Whole Tissue Application: IHC Frozen A tissue-specific role for intraflagellar transport genes during craniofacial development Authors: EN Schock, JN Struve, CF Chang, TJ Williams, J Snedeker, AC Attia, RW Stottmann, SA Brugmann PLoS ONE, 2017;12(3):e0174206. Species: Mouse Sample Type: Whole Tissue Application: IHC Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through PDGF and Notch signaling Authors: T Liang, L Zhu, W Gao, M Gong, J Ren, H Yao, K Wang, D Shi FEBS Open Bio, 2017;7(11):1722-1736. Species: Human Sample Type: Whole Cells Application: Neut Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis. Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G J Biol Chem, 2014;289(33):22835-49. Species: Human Sample Type: Cell Lysates Application: WB Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration. Authors: Yuan, Liangpin, Zhang, Hongying, Liu, Jingbo, Rubin, Joshua B, Cho, Yoon-Jae, Shu, Hui Kuo, Schniederjan, Matthew, MacDonald, Tobey J Mol Cancer, 2013;12(0):18. Species: Human Sample Type: Whole Cells Application: Neut PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012;125(0):2276-87. Species: Human Sample Type: Whole Cells Application: Neut Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase. Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM J. Biol. Chem., 2009;284(24):16693-703. Species: Human Sample Type: Cell Lysates Application: WB Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model. Authors: Miyake T, Aoki M, Morishita R Cardiovasc. Res., 2008;79(4):706-14. Species: Rabbit Sample Type: Tissue Homogenates Application: WB Pancreatic stellate cells: partners in crime with pancreatic cancer cells. Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV Cancer Res., 2008;68(7):2085-93. Species: Human Sample Type: Whole Cells Application: ICC C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium. Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF Blood, 2007;109(10):4264-71. Species: Human Sample Type: Whole Cells Application: Neut Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Whole Cells Application: Neut PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Authors: Li R, Maminishkis A, Wang FE, Miller SS Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32. Species: Human Sample Type: Whole Cells Application: Neut Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration. Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG Eur J Vasc Endovasc Surg, 2006;31(5):516-22. Species: Human Sample Type: Whole Tissue Application: IHC Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells. Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A Blood, 2006;107(5):1896-902. Species: Human Sample Type: Whole Cells Application: Neut Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis. Authors: Campbell M, Allen WE, Silversides JA, Trimble ER Diabetes, 2003;52(2):519-26. Species: Human Sample Type: Whole Cells Application: ICC Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty. Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH Am. J. Pathol., 2003;163(3):869-78. Species: Rabbit Sample Type: Cell Lysates Application: WB Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Description Application Cat# Citations Images

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 17

Expedite Your Research

Award-Winning Interactive Pathways

Posters, Brochures, Whitepapers & Reference Guides

Experts in Reagent Quality

Our People are Our Greatest Asset

You are here

Human PDGF R beta Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Basic

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Acidic

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Rabbit Anti-Goat IgG Biotinylated Antibody

Chicken Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Order Details

Feedback

Reconstitution Calculator