Human PDGF R beta Antibody

(15 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PDGF R beta is observed, and less than 1% cross-reactivity with recombinant human PDGF sR alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
    Leu33-Phe530 (Glu241Asp)
    Accession # P09619
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR)
  • Flow Cytometry
    2.5 µg/106 cells
    BUD‑8 human fibroblast cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize PDGF R beta inhibition of PDGF-BB-dependent proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 2 μg/mL Recombinant Human PDGF R beta Fc Chimera and 4 ng/mL Recombinant Human PDGF-BB.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
PDGF R beta  in Human Breast Cancer Tissue.

PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

PDGF R beta  Inhibition of PDGF-BB-dependent Cell Proliferation and Neutralization by Human PDGF R beta Antibody.

Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220‑BB) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND50 is typically
10‑40 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

  • References:
    1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.
  • Long Name:
    Platelet-derived Growth Factor Receptor beta
  • Entrez Gene IDs:
    5159 (Human); 18596 (Mouse); 24629 (Rat)
  • Alternate Names:
    beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. A tissue-specific role for intraflagellar transport genes during craniofacial development
    Authors: EN Schock, JN Struve, CF Chang, TJ Williams, J Snedeker, AC Attia, RW Stottmann, SA Brugmann
    PLoS ONE, 2017;12(3):e0174206.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia
    Authors: SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro
    PLoS ONE, 2017;12(8):e0182265.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.
    Authors: Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G
    J Biol Chem, 2014;289(33):22835-49.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration.
    Authors: Yuan, Liangpin, Zhang, Hongying, Liu, Jingbo, Rubin, Joshua B, Cho, Yoon-Jae, Shu, Hui Kuo, Schniederjan, Matthew, MacDonald, Tobey J
    Mol Cancer, 2013;12(0):18.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
    J. Cell. Sci., 2012;125(0):2276-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase.
    Authors: Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM
    J. Biol. Chem., 2009;284(24):16693-703.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model.
    Authors: Miyake T, Aoki M, Morishita R
    Cardiovasc. Res., 2008;79(4):706-14.
    Species: Rabbit
    Sample Type: Tissue Homogenates
    Application: WB
  8. Pancreatic stellate cells: partners in crime with pancreatic cancer cells.
    Authors: Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV
    Cancer Res., 2008;68(7):2085-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines.
    Authors: Li R, Maminishkis A, Wang FE, Miller SS
    Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. C-KIT, by interacting with the membrane-bound ligand, recruits endothelial progenitor cells to inflamed endothelium.
    Authors: Dentelli P, Rosso A, Balsamo A, Colmenares Benedetto S, Zeoli A, Pegoraro M, Camussi G, Pegoraro L, Brizzi MF
    Blood, 2007;109(10):4264-71.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  12. Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
    Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
    Eur J Vasc Endovasc Surg, 2006;31(5):516-22.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  13. Heterodimerization of FGF-receptor 1 and PDGF-receptor-alpha: a novel mechanism underlying the inhibitory effect of PDGF-BB on FGF-2 in human cells.
    Authors: Faraone D, Aguzzi MS, Ragone G, Russo K, Capogrossi MC, Facchiano A
    Blood, 2006;107(5):1896-902.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  14. Glucose-induced phosphatidylinositol 3-kinase and mitogen-activated protein kinase-dependent upregulation of the platelet-derived growth factor-beta receptor potentiates vascular smooth muscle cell chemotaxis.
    Authors: Campbell M, Allen WE, Silversides JA, Trimble ER
    Diabetes, 2003;52(2):519-26.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
    Authors: Nili N, Cheema AN, Giordano FJ, Barolet AW, Babaei S, Hickey R, Eskandarian MR, Smeets M, Butany J, Pasterkamp G, Strauss BH
    Am. J. Pathol., 2003;163(3):869-78.
    Species: Rabbit
    Sample Type: Cell Lysates
    Application: WB
Expand to show all 15 Citations
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