Human Perilipin-3 Antibody Summary
Accession # O60664
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Perilipin‑3 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Perilipin‑3 in Human Placenta. Perilipin-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
M6PRBP1 (mannose 6-phosphate receptor binding protein), gene name PLIN3 (Perilipin-3), also called TIP47 (tail interacting protein of 47 kDa) or PP17 (placental protein 17), is a 47 kDa intracellular transport protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. The 434 amino acid (aa) human Perilipin‑3, which shares approximately 75% aa sequence identity with mouse and rat Perilipin-3, contains a four-helix bundle thought to interact with membranes, and may be phosphorylated and acetylated. A 251 aa form (isoform A) has an alternate start site at aa 184 of the 434 aa form (isoform B). Perilipin-3 is normally found predominantly in the cytoplasm. It is required for uptake of mannose-6 phosphate receptors into endosomes. In the presence of excess lipids, such as in macrophage-derived foam cells, Perilipin-3 may transfer free fatty acids to lipid droplets, promoting their growth.
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