Human Phospho-CREB (S133) Antibody

Catalog # Availability Size / Price Qty
MAB6906
MAB6906-SP
Detection of Human Phospho-CREB (S133) by Western Blot.
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Human Phospho-CREB (S133) Antibody Summary

Species Reactivity
Human
Specificity
Detects human CREB when phosphorylated at S133.
Source
Monoclonal Mouse IgG2A Clone # 702710
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human CREB S133 site 
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-CREB (S133) by Western Blot. View Larger

Detection of Human Phospho-CREB (S133) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) with a 30 minute recovery. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-CREB (S133) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-CREB (S133) in HeLa Human Cell Line. View Larger

Phospho-CREB (S133) in HeLa Human Cell Line. CREB phosphorylated at A133 was detected in immersion fixed Hela human cervical epithelial carcinoma cells, unstimulated (lower panel) and stimulated (upper panel) with PMA, using Mouse Anti-Human Phospho-CREB (S133) Monoclonal Antibody (Catalog # MAB6906) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB) belongs to the bZIP superfamily of transcription factors, containing a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. When phosphorylated at Ser133, CREB also binds the coactivator CREB binding protein (CBP), which enhances transcription by acetylating histones to facilitate chromatin unraveling.

Long Name
cAMP Response Element-binding Protein
Entrez Gene IDs
1385 (Human); 12912 (Mouse)
Alternate Names
active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

Reviews for Human Phospho-CREB (S133) Antibody

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Human Phospho-CREB (S133) Antibody
By Anonymous on 08/23/2018
Application: WB Sample Tested: Human fibroblast Species: Human