Detection of Human Phospho-Lck (Y394) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-Lck (Y394) at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho‑Lck (Y394) in Jurkat Human Cell Line. Lck phosphorylated at Y394 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-Lck (Y394) Monoclonal Antibody (Catalog # MAB7500) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lck (p56lck) is a 56 kDa
cytosolic phosphoprotein within the Src family of non-receptor tyrosine
kinases. The 509 amino acid (aa) human Lck contains an SH3 domain (aa 61-121),
an SH2 domain (aa 127-224) and a protein kinase domain (aa 251-259). A short
(363 aa) isoform contains alternate sequence starting at aa 348, while a 539 aa
isoform contains inserted sequence after aa 321. Lck interacts with T cell CD4
and CD8 molecules and plays a pivotal role in regulating T cell activation. It
can be overexpressed in cancers and functions as an oncogene. Y394 is the major
site for autophosphorylation that enhances kinase activity, while Y505 is the
major phosphorylation site for negative regulation.
Lymphocyte Protein Tyrosine Kinase
Entrez Gene IDs:
3932 (Human); 16818 (Mouse); 313050 (Rat)
EC 2.7.10; EC 188.8.131.52; Hck-3; Lcktkr; Leukocyte C-terminal Src kinase; LSK; LSTRA; Lymphocyte cell-specific protein-tyrosine kinase; lymphocyte-specific protein tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; p56-LCK; pp58lck; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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