Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 776503
View all SLP-76/LCP2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human SLP-76/LCP2 Y145 site
Accession # Q13094

Specificity

Detects human Phospho-SLP-76/LCP2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Phospho-SLP‑76/LCP2 (Y145) Antibody

Detection of Human Phospho-SLP-76/LCP2 antibody by Western Blot.

Detection of Human Phospho-SLP‑76/LCP2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Phospho-SLP-76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-SLP-76/LCP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-SLP-76/LCP2 (Y145) antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

Phospho-SLP-76/LCP2 (Y145) in Jurkat Human Cell Line.

SLP-76/LCP2 phosphorylated at Y145 was detected in immersion fixed Jurkat human acute T cell leukemia cells treated with (upper panel) or without (lower panel) Pervanadate using Mouse Anti-Human Phospho-SLP-76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Phospho-Human SLP‑76/LCP2 (Y145) antibody by Simple WesternTM.

Detection of Phospho-Human SLP‑76/LCP2 (Y145) by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Human SLP‑76/LCP2 (Y145) at approximately 76-80 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Phospho-SLP‑76/LCP2 (Y145) Monoclonal Antibody (Catalog # MAB7474). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Phospho-SLP‑76/LCP2 (Y145) Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Pervanadate

Simple Western

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLP-76/LCP2

SLP-76 is a 533 amino acid (aa), 76 kDa tyrosine phosphorylated intracellular docking protein with a single SH2 recognition domain. It is expressed in T cells, platelets, neutrophils, NK and mast cells, and is required for progression of T cells to double positive stage in the thymus. Tyrosine phosphorylation is required for interaction with the signaling proteins VAV, NCK, PLC gamma, ITK, and ZAP70. SLP-76 may be phosphorylated by ZAP70 leading to NF-AT and IL2 gene activation. Human SLP-76 shares 83% and 85% aa sequence identity with mouse and rat SLP-76, respectively.

Long Name

SH2 Domain-containing Leukocyte Protein of 76 kDa

Alternate Names

LCP2, SLP76

Entrez Gene IDs

3937 (Human); 16822 (Mouse); 155918 (Rat)

Gene Symbol

LCP2

UniProt

Q13094

Additional SLP-76/LCP2 Products

Product Documents for Human Phospho-SLP‑76/LCP2 (Y145) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-SLP‑76/LCP2 (Y145) Antibody

For research use only

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Protocols

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