Human PIEZO2 Antibody

Catalog #: MAB11559 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11559-100
MAB11559-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PIEZO2 Antibody Summary

Species Reactivity

Human

Specificity

Detects a synthetic peptide specific for Piezo2 around amino acid 2115 in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1081503

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

PIEZO2 containing synthetic peptide
Accession # Q9H5I5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

0.5-15 µg/mL

Immersion fixed paraffin-embedded sections of human breast

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of PIEZO2 in Human Breast. PIEZO2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human PIEZO2 Monoclonal Antibody (Catalog # MAB11559) at 0.05 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIEZO2

PIEZO2 is an 318 kDa mechanosensitive nonselective cation channel, found in cell types that typically respond to physical touch. PIEZO2 is an essential mechanotransducer for touch, proprioception, and interoception. PIEZO2 is important for aspects of breathing, bladder control, blood pressure regulation and many other processes since it is expressed in several classes of jugular and nodose ganglia neurons. Loss of function of PIEZO2 results in profound lack of proprioception and is diagnosed as a rare disease caused PIEZO2 deficiency syndrome.

References

Wu J, Lewis AH, Grandl J. Touch, Tension, and Transduction - The Function and Regulation of Piezo Ion Channels. Trends Biochem Sci. 2017 Jan;42(1):57-71. doi: 10.1016/j.tibs.2016.09.004. Epub 2016 Oct 12. PMID: 27743844; PMCID: PMC5407468.
Szczot M, Nickolls AR, Lam RM, Chesler AT. The Form and Function of PIEZO2. Annu Rev Biochem. 2021 Jun 20;90:507-534. doi: 10.1146/annurev-biochem-081720-023244. PMID: 34153212; PMCID: PMC8794004.

Long Name

PIEZO2

Entrez Gene IDs

63895 (Human); 667742 (Mouse); 307380 (Rat)

Alternate Names

C18orf30; C18orf58; DA3; DA5; DAIPT; FAM38B; FAM38B2; family with sequence similarity 38, member A pseud; family with sequence similarity 38, member B; family with sequence similarity 38, member B2; FLJ23144; FLJ23403; FLJ25916; FLJ34907; FLJ37734; FLJ45725; HsT748; HsT771; MWKS; PIEZO2; piezo-type mechanosensitive ion channel component ; piezo-type mechanosensitive ion channel component 2; protein PIEZO2