Human PKM2 Antibody

Catalog # Availability Size / Price Qty
Detection  of Human PKM2 by Western Blot.
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Product Details
Supplemental Products

Human PKM2 Antibody Summary

Species Reactivity
Detects human PKM2 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 945103
Protein A or G purified from hybridoma culture supernatant
E.coli-derived recombinant human PKM2
Accession # P14618
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/106 cells
See below
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human PKM2 by Western Blot. View Larger

Detection of Human PKM2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PKM2 Monoclonal Antibody (Catalog # MAB72443) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PKM2 in  HeLa Human Cell Line. View Larger

PKM2 in HeLa Human Cell Line. PKM2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human PKM2 Monoclonal Antibody (Catalog # MAB72443) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKM2

PKM2 (Pyruvate Kinase isoenzyme M2; also p58, OIP3, THBP1 and CTHBP) is a 58-60 kDa member of the PK family of enzymes. It is widely expressed, being found both intracellularly and in blood, and represents the more common splice variant of the PKM gene. PKM2 generates ATP and pyruvate by catalyzing the transfer of a phosphoryl group from PEP to ADP. Thus, when active, PKM2 promotes energy production and glycolysis. PKM2 exists as a marginally active monomer, with full activity achieved through homotetramerization. Notably, in tumor cells, select oncogenes appear to induce PKM2 homodimerization which limits PKM2 activity. PKM2 is known to be regulated by the binding of T3 and Fru-1,6-bisP. Human PKM2 is 531 amino acids (aa) in length. It contains a catalytic region (aa 43‑527) plus four utilized Ser/Thr and Tyr phosphorylation sites, respectively. PKM1 is another PKM gene splice variant that shows a 45 aa substitution for aa 389‑433 of PKM2. This variant shows limited expression (striated muscle) and hyperbolic Michaelis-Menten kinetics. There are additional isoform variants of PKM2 that show either a deletion of aa 59‑132, or a 67 aa substitution for aa 1‑82. Over aa 434‑531, human PKM2 shares 95% aa sequence identity with mouse PKM2.

Long Name
Pyruvate Kinase, Muscle
Entrez Gene IDs
5315 (Human); 18746 (Mouse); 25630 (Rat)
Alternate Names
CTHBP; Cytosolic thyroid hormone-binding protein; MGC3932; OIP3; OIP-3; OIP3EC; Opa-interacting protein 3; p58; PK, muscle type; PK2; PK3; PK3PKM; PKM2; Pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; pyruvate kinase, muscle; TCB; THBP1; THBP1p58; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control


Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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