Human PNR/NR2E3 Antibody

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PP-H7223-00

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Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence
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Product Details
Citations (10)
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Human PNR/NR2E3 Antibody Summary

Species Reactivity
Human
Source
Monoclonal Mouse IgG2a Clone # H7223
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.
Immunohistochemistry
5-15 µg/mL
Perfusion fixed paraffin-embedded sections of rat retina (outer nuclear layer)
Direct ELISA
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence Coexpression of Rxrg and Nr2e3 in the embryonic mouse retina. (A-H) E14.5 mouse retinas electroporated with either the cNr2e3Enh2 or cNr2e3Enh3 plasmid, cultured ex vivo for 2 days, and processed for immunofluorescence confocal imaging of EGFP (green, chicken antibody), Nr2e3 (red, mouse antibody), Rxrg (white, rabbit antibody), and DAPI. Panels in A-H represent maximum projections of z-stacks with the depicted channel shown at the top of the column. The merge column has EGFP, Nr2e3, and Rxrg signals. (A’-H’) Magnified single z-plane images with the signals for (A’,E’) EGFP, (B’,F’) Nr2e3, (C’,G’) Rxrg, and (D’,H’) DAPI. White arrows point to GFP+ cells that also express Nr2e3 and Rxrg. Yellow arrows point to GFP+ cells that express Rxrg, but not Nr2e3. (I) A graph of the average percentage of GFP+ cells when driven by the cNr2e3Enh2 or cNr2e3Enh3 elements that express Nr2e3, Rxrg, or both Nr2e3 and Rxrg. (J-L) Maximum projection of a z-stack image of a E17.5 mouse retina processed for immunofluorescent detection of Nr2e3 (J, green) and Rxrg (K, red) or both (L, Merge). (J’-L’) Magnified single z-plane images of the same area visualized for signals for Nr2e3 (J’), Rxrg (K’), or DAPI (L’). (M) A graph of the average percentage of Nr2e3, Rxrg double-positive (D.P.) cells out of the total Nr2e3+ population (left bar) or the total Rxrg+ population (right bar). In both graphs N ≥ 3 biological replicates. Error bars represent standard error of the mean. All images are oriented with the scleral side of the retina at the top of the image. Scale bar in A represents 20 μm and applies to A-L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30466480), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse PNR/NR2E3 by Immunocytochemistry/Immunofluorescence Coexpression of Rxrg and Nr2e3 in the embryonic mouse retina. (A-H) E14.5 mouse retinas electroporated with either the cNr2e3Enh2 or cNr2e3Enh3 plasmid, cultured ex vivo for 2 days, and processed for immunofluorescence confocal imaging of EGFP (green, chicken antibody), Nr2e3 (red, mouse antibody), Rxrg (white, rabbit antibody), and DAPI. Panels in A-H represent maximum projections of z-stacks with the depicted channel shown at the top of the column. The merge column has EGFP, Nr2e3, and Rxrg signals. (A’-H’) Magnified single z-plane images with the signals for (A’,E’) EGFP, (B’,F’) Nr2e3, (C’,G’) Rxrg, and (D’,H’) DAPI. White arrows point to GFP+ cells that also express Nr2e3 and Rxrg. Yellow arrows point to GFP+ cells that express Rxrg, but not Nr2e3. (I) A graph of the average percentage of GFP+ cells when driven by the cNr2e3Enh2 or cNr2e3Enh3 elements that express Nr2e3, Rxrg, or both Nr2e3 and Rxrg. (J-L) Maximum projection of a z-stack image of a E17.5 mouse retina processed for immunofluorescent detection of Nr2e3 (J, green) and Rxrg (K, red) or both (L, Merge). (J’-L’) Magnified single z-plane images of the same area visualized for signals for Nr2e3 (J’), Rxrg (K’), or DAPI (L’). (M) A graph of the average percentage of Nr2e3, Rxrg double-positive (D.P.) cells out of the total Nr2e3+ population (left bar) or the total Rxrg+ population (right bar). In both graphs N ≥ 3 biological replicates. Error bars represent standard error of the mean. All images are oriented with the scleral side of the retina at the top of the image. Scale bar in A represents 20 μm and applies to A-L. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30466480), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: PNR/NR2E3

Photoreceptor-specific Nuclear Receptor (PNR, RNR; NR2E3) is a member of the orphan nuclear receptor superfamily. PNR is expressed in the retina and plays a role in retinal photoreceptor cell differentiation and degeneration.

Long Name
Photoreceptor-specific Nuclear Receptor
Entrez Gene IDs
10002 (Human)
Alternate Names
NR2E3; Nuclear receptor subfamily 2 group E member 3; nuclear receptor subfamily 2, group E, member 3; photoreceptor-specific nuclear receptor; PNR; PNRESCS; rd7; Retina-specific nuclear receptor; RNR; RP37MGC49976

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Citations for Human PNR/NR2E3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Lamin A upregulation reorganizes the genome during rod photoreceptor degeneration
    Authors: Herrera, I;Fernandes, JAL;Shir-Mohammadi, K;Levesque, J;Mattar, P;
    Cell death & disease
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Automating iPSC generation to enable autologous photoreceptor cell replacement therapy
    Authors: LR Bohrer, NE Stone, NK Mullin, AP Voigt, KR Anfinson, JL Fick, V Luangphakd, B Hittle, K Powell, GF Muschler, RF Mullins, EM Stone, BA Tucker
    Journal of Translational Medicine, 2023-02-28;21(1):161.
    Species: Human
    Sample Types: Organoid
    Applications: IHC
  3. Biocompatibility of Human Induced Pluripotent Stem Cell-Derived Retinal Progenitor Cell Grafts in Immunocompromised Rats
    Authors: IC Han, LR Bohrer, KN Gibson-Cor, LA Wiley, A Shrestha, BE Harman, C Jiao, EH Sohn, R Wendland, BN Allen, KS Worthingto, RF Mullins, EM Stone, BA Tucker
    Cell Transplantation, 2022-01-01;31(0):9636897221104.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Setd1a Plays Pivotal Roles for the Survival and Proliferation of Retinal Progenitors via Histone Modifications of Uhrf1
    Authors: X Deng, T Iwagawa, M Fukushima, Y Suzuki, S Watanabe
    Investigative Ophthalmology & Visual Science, 2021-05-03;62(6):1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC-derived retinal organoids
    Authors: H Xie, W Zhang, M Zhang, T Akhtar, Y Li, W Yi, X Sun, Z Zuo, M Wei, X Fang, Z Yao, K Dong, S Zhong, Q Liu, Y Shen, Q Wu, X Wang, H Zhao, J Bao, K Qu, T Xue
    Sci Adv, 2020-02-07;6(6):eaay5247.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Identification of Genes With Enriched Expression in Early Developing Mouse Cone Photoreceptors
    Authors: Diego F. Buenaventura, Adrianne Corseri, Mark M. Emerson
    Investigative Opthalmology & Visual Science
  7. Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1
    Authors: Nathalie Jean-Charles, Diego F. Buenaventura, Mark M. Emerson
    Neural Development
  8. Requirements for Neurogenin2 during mouse postnatal retinal neurogenesis
    Authors: Angelica M. Kowalchuk, Kate A. Maurer, Farnaz Shoja-Taheri, Nadean L. Brown
    Developmental Biology
  9. Small molecule Photoregulin3 prevents retinal degeneration in the RhoP23H mouse model of retinitis pigmentosa
    Authors: PA Nakamura, AA Shimchuk, S Tang, Z Wang, K DeGolier, S Ding, TA Reh
    Elife, 2017-11-17;6(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  10. Transplantation of photoreceptors derived from human Muller glia restore rod function in the P23H rat.
    Authors: Jayaram H, Jones M, Eastlake K, Cottrill P, Becker S, Wiseman J, Khaw P, Limb G
    Stem Cells Transl Med, 2014-01-29;3(3):323-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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