Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 253717
View all BMP-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑2
Ala22-Lys281 (Arg245Ser, Arg279Ser)
Accession # P12643

Specificity

Detects the pro region of the human BMP-2 precursor in direct ELISAs and Western blots. This antibody does not recognize mature recombinant human BMP‑2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Pro-BMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Western Blot

1 µg/mL
Sample: Recombinant Human BMP‑2 (Pro Region)

Reviewed Applications

Read 1 review rated 5 using MAB2260 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2

BMP-2, a member of the transforming growth factor beta (TGF-beta ) superfamily, is synthesized as an inactive precursor protein. The N-terminal pro region is removed via proteolytic cleavage to liberate a free 114 amino acid C-terminal fragment that forms a disulfide-linked active BMP-2 homodimer. BMP-2 modulates growth and differentiation of many cell types through its interaction with heterodimeric receptors composed of BMPR-II complexed with BMPR-IA or -IB.

Long Name

Bone Morphogenetic Protein 2

Alternate Names

BDA2, BMP2, SSFSC

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

P12643

Additional BMP-2 Products

Product Documents for Human Pro-BMP‑2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pro-BMP‑2 Antibody

For research use only

Citations for Human Pro-BMP‑2 Antibody

Customer Reviews for Human Pro-BMP‑2 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human Pro-BMP‑2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Pancreatic cancer tissue
    Species: Human
    Verified Customer | Posted 06/17/2022
    Human Pro-BMP‑2 Antibody MAB2260

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Protocols

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FAQs

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